Human PYCRL Recombinant Protein (RPPB2234)
- SKU:
- RPPB2234
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q53H96
- Research Area:
- Enzymes
Description
| Product Name: | Human PYCRL Recombinant Protein |
| Product Code: | RPPB2234 |
| Size: | 10µg |
| Species: | Human |
| Target: | PYCRL |
| Synonyms: | Pyrroline-5-carboxylate reductase 3, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase-like protein, PYCRL. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PYCRL solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 50% glycerol and 2mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMAAAEPS PRRVGFVGAG RMAGAIAQGL IRAGKVEAQH ILASAPTDRN LCHFQALGCR TTHSNQEVLQ SCLLVIFATK PHVLPAVLAE VAPVVTTEHI LVSVAAGVSL STLEELLPPN TRVLRVLPNL PCVVQEGAIV MARGRHVGSS ETNLLQHLLE ACGRCEEVPE AYVDIHTGLS GSGVAFVCAF SEALAEGAVK MGMPSSLAHR IAAQTLLGTA KMLLHEGQHP AQLRSDVCTP GGTTIYGLHA LEQGGLRAAT MSAVEAATCR AKELSRK |
Pyrroline-5-Carboxylate Reductase Like (PYCRL) is a member of the pyrroline-5-carboxylate reductase family and acts as a homodecamer. PYCRL plays a key role in proline bio-synthesis. Proline serves as a non-enzymatic antioxidant to reduce damage caused by reactive oxygen species (ROS) in microorganisms, animals and plants. In the final stage of proline biosynthesis, PYCRL catalyzes the reduction of aldehyde dehydrogenase 4A1 (ALDH4A1) to proline with NAD(P)H as the cofactor.
PYCRL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 297 amino acids (1-274) and having a molecular mass of 31kDa.PYCRL is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PYCRL: Belongs to the pyrroline-5-carboxylate reductase family. |
| UniProt Protein Details: | Protein type:Hydrolase; Oxidoreductase; EC 1.5.1.2; Amino Acid Metabolism - arginine and proline Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: cytosol Molecular Function:protein binding; pyrroline-5-carboxylate reductase activity Biological Process: amino acid biosynthetic process |
| UniProt Code: | Q53H96 |
| NCBI GenInfo Identifier: | 727863586 |
| NCBI Gene ID: | 65263 |
| NCBI Accession: | Q53H96.3 |
| UniProt Secondary Accession: | Q53H96,Q8N3N9, Q96HX4, Q9H896, B3KMB5, B4DVT6, H0Y6C3 |
| UniProt Related Accession: | Q53H96 |
| Molecular Weight: | 26,440 Da |
| NCBI Full Name: | Pyrroline-5-carboxylate reductase 3 |
| NCBI Synonym Full Names: | pyrroline-5-carboxylate reductase-like |
| NCBI Official Symbol: | PYCRL�� |
| NCBI Official Synonym Symbols: | PYCR3�� |
| NCBI Protein Information: | pyrroline-5-carboxylate reductase 3 |
| UniProt Protein Name: | Pyrroline-5-carboxylate reductase 3 |
| UniProt Synonym Protein Names: | Pyrroline-5-carboxylate reductase-like protein |
| UniProt Gene Name: | PYCRL�� |
| UniProt Entry Name: | P5CR3_HUMAN |
