Human RBP1 (Retinol Binding Protein 1, Cellular) CLIA Kit
The Human RBP1 (Retinol-Binding Protein 1) cellular CLIA Kit is specifically designed for the accurate quantification of RBP1 levels in human cell samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.RBP1 is a key protein involved in cellular retinol transport and metabolism, playing a critical role in maintaining healthy vision and skin, as well as supporting overall immune function.
Abnormal levels of RBP1 have been linked to various health conditions, making it a valuable biomarker for studying diseases such as vitamin A deficiency, liver disease, and certain cancers.With its advanced technology and reliable performance, the Human RBP1 cellular CLIA Kit is an essential tool for researchers looking to accurately measure RBP1 levels and further understand its impact on human health.
Product Name:
Human RBP1 (Retinol Binding Protein 1, Cellular) CLIA Kit
SKU:
HUES00899
Target:
Human RBP1 (Retinol Binding Protein 1, Cellular)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human RBP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RBP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RBP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human RBP1. You can calculate the concentration of Human RBP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
88-99
94-107
Average (%)
103
93
102
1:4
Range (%)
93-106
88-100
93-105
Average (%)
99
95
100
1:8
Range (%)
91-104
95-110
98-114
Average (%)
98
101
104
1:16
Range (%)
92-103
97-113
84-97
Average (%)
97
104
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-117
107
EDTA plasma (n=5)
96-110
101
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.84
201.37
899.82
90.49
195.37
893.92
Standard deviation
9.64
22.11
91.33
9.29
22.7
79.74
C V (%)
9.85
10.98
10.15
10.27
11.62
8.92
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human RBP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RBP1 in samples. No significant cross-reactivity or interference between Human RBP1 and analogues was observed.
