Human RPIA Recombinant Protein (RPPB2256)
- SKU:
- RPPB2256
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P49247
- Research Area:
- Enzymes
Description
| Product Name: | Human RPIA Recombinant Protein |
| Product Code: | RPPB2256 |
| Size: | 10µg |
| Species: | Human |
| Target: | RPIA |
| Synonyms: | Ribose-5-phosphate isomerase, Phosphoriboisomerase, RPIA, RPI. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The RPIA solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT,40% glycerol, 200mM NaCl, 2mM EDTA and 0.2mM PMSF. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MQRPGPFSTL YGRVLAPLPG RAGGAASGGG GNSWDLPGSH VRLPGRAQSG TRGGAGNTST SCGDSNSICP APSTMSKAEE AKKLAGRAAV ENHVRNNQVL GIGSGSTIVH AVQRIAERVK QENLNLVCIP TSFQARQLIL QYGLTLSDLD RHPEIDLAID GADEVDADLN LIKGGGGCLT QEKIVAGYAS RFIVIADFRK DSKNLGDQWH KGIPIEVIPM AYVPVSRAVS QKFGGVVELR MAVNKAGPVV TDNGNFILDW KFDRVHKWSE VNTAIKMIPG VVDTGLFINM AERVYFGMQD GSVNMREKPF C |
Ribose-5-phosphate isomerase (RPIA) is a member of the ribose 5-phosphate isomerase family. RPIA is an enzyme which catalyzes the conversion between ribose-5-phosphate (R5P) and ribulose-5-phosphate (Ru5P). RPI occurs as 2 separate proteins forms, termed RPIA and RPIB. RPIA has a vital role in the metabolism of plants and animals, as it is involved in the Calvin cycle which takes place in plants, and the pentose phosphate pathway which occurs in plants as well as animals.
RPIA Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 331 amino acids (1-311) and having a molecular mass of 35.4kDa.RPIA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RPIA: Defects in RPIA are the cause of ribose 5-phosphate isomerase deficiency (RPID). A patient has been described with a deficiency of ribose 5-phosphate isomerase who presented with leukoencephalopathy and peripheral neuropathy. Proton magnetic resonance spectroscopy of the brain revealed a highly elevated level of the polyols ribitol and D-arabitol, which were subsequently also found in high concentrations in body fluids. Deficient activity of RPIA, one of the pentose phosphate pathway enzymes, has been demonstrated in fibroblasts. Belongs to the ribose 5-phosphate isomerase family. |
| UniProt Protein Details: | Protein type:EC 5.3.1.6; Isomerase; Carbohydrate Metabolism - pentose phosphate pathway Chromosomal Location of Human Ortholog: 2p11.2 Cellular Component: cytosol Molecular Function:protein binding; ribose-5-phosphate isomerase activity Biological Process: pentose-phosphate shunt Disease: Ribose 5-phosphate Isomerase Deficiency |
| NCBI Summary: | The protein encoded by this gene is an enzyme, which catalyzes the reversible conversion between ribose-5-phosphate and ribulose-5-phosphate in the pentose-phosphate pathway. This gene is highly conserved in most organisms. The enzyme plays an essential role in the carbohydrate metabolism. Mutations in this gene cause ribose 5-phosphate isomerase deficiency. A pseudogene is found on chromosome 18. [provided by RefSeq, Mar 2010] |
| UniProt Code: | P49247 |
| NCBI GenInfo Identifier: | 156637353 |
| NCBI Gene ID: | 22934 |
| NCBI Accession: | P49247.3 |
| UniProt Secondary Accession: | P49247,Q541P9, Q96BJ6, |
| UniProt Related Accession: | P49247 |
| Molecular Weight: | 33,269 Da |
| NCBI Full Name: | Ribose-5-phosphate isomerase |
| NCBI Synonym Full Names: | ribose 5-phosphate isomerase A |
| NCBI Official Symbol: | RPIA�� |
| NCBI Official Synonym Symbols: | RPI; RPIAD�� |
| NCBI Protein Information: | ribose-5-phosphate isomerase |
| UniProt Protein Name: | Ribose-5-phosphate isomerase |
| UniProt Synonym Protein Names: | Phosphoriboisomerase |
| Protein Family: | Ribose-5-phosphate isomerase |
| UniProt Gene Name: | RPIA�� |
| UniProt Entry Name: | RPIA_HUMAN |
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