The Human SDC4 (Syndecan-4) CLIA Kit is a highly reliable and sensitive assay designed for the accurate detection of Syndecan-4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides researchers with precise and reproducible results, making it an ideal tool for a wide range of applications in the field of biomedical research.Syndecan-4 is a cell surface heparan sulfate proteoglycan that plays a crucial role in cell adhesion, migration, and signaling. It is involved in various physiological processes such as wound healing, inflammation, and tissue repair, making it a valuable biomarker for studying these processes and potentially identifying therapeutic targets.
By utilizing the Human SDC4 CLIA Kit, researchers can gain valuable insights into the role of Syndecan-4 in disease pathogenesis, particularly in conditions such as cancer, cardiovascular diseases, and inflammatory disorders. With its high sensitivity and specificity, this kit offers a reliable platform for accurately measuring Syndecan-4 levels, enabling researchers to advance their understanding of the molecular mechanisms underlying these diseases.
Product Name:
Human SDC4 (Syndecan 4) CLIA Kit
SKU:
HUES00943
Target:
Human SDC4 (Syndecan 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SDC4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SDC4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SDC4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SDC4. You can calculate the concentration of Human SDC4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-120
99-112
91-107
Average (%)
110
105
98
1:4
Range (%)
100-115
99-115
83-95
Average (%)
106
105
90
1:8
Range (%)
84-98
89-103
102-117
Average (%)
90
95
108
1:16
Range (%)
98-110
98-115
96-110
Average (%)
103
106
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
103
EDTA plasma (n=5)
100-115
107
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
2.42
8.32
0.97
2.2
9.03
Standard deviation
0.11
0.24
0.71
0.12
0.18
0.73
C V (%)
11.22
9.92
8.53
12.37
8.18
8.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SDC4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SDC4 in samples. No significant cross-reactivity or interference between Human SDC4 and analogues was observed.
