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Human SORD Recombinant Protein (RPPB2278)

SKU:
RPPB2278
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q00796
Research Area:
Enzymes
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Description

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Product Name:Human SORD Recombinant Protein
Product Code:RPPB2278
Size:25µg
Species:Human
Target:SORD
Synonyms:EC 1.1.1.14, SORD1,SORD, L-iditol 2-dehydrogenase, DHSO, Sorbitol Dehydrogenase.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:SORD protein solution (0.5mg/ml) is formulated in 20mM Tris-HCl pH-8, 0.2M NaCl, 5mM DTT & 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR YNLSPSIFFC ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGALIEPLSV GIHACRRGGV TLGHKVLVCG AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGQL GCKPEVTIEC TGAEASIQAG IYATRSGGTL VLVGLGSEMT TVPLLHAAIR EVDIKGVFRY CNTWPVAISM LASKSVNVKP LVTHRFPLEK ALEAFETFKK GLGLKIMLKC DPSDQNP
Biological Activity:Specific activity is > 0.2 units/mg, in which one unit will convert 1.0 umole of D-fructose to D-sorbitol per minute at pH 7.5 at 25�C.

SORD enzyme is part of of the zinc-containing alcohol dehydrogenase family that is broadly expressed in kidney and in the lens eye. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses.

SORD Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 377 amino acids (1-357 a.a.) and having a molecular mass of 40.4 kDa. SORD protein is fused to a 20 amino acid His tag at N-terminus and is purified by standard chromatography.

UniProt Protein Function:SORD: Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. Belongs to the zinc-containing alcohol dehydrogenase family.
UniProt Protein Details:

Protein type:EC 1.1.1.14; Oxidoreductase; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 15q15.3

Cellular Component: extracellular space; membrane; mitochondrial membrane

Molecular Function:zinc ion binding; L-iditol 2-dehydrogenase activity; carbohydrate binding; NAD binding

Biological Process: sperm motility; L-xylitol metabolic process; L-xylitol catabolic process; glucose metabolic process; fructose biosynthetic process; sorbitol catabolic process

NCBI Summary:Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]
UniProt Code:Q00796
NCBI GenInfo Identifier:292495088
NCBI Gene ID:6652
NCBI Accession:Q00796.4
UniProt Related Accession:Q00796
Molecular Weight:
NCBI Full Name:Sorbitol dehydrogenase
NCBI Synonym Full Names:sorbitol dehydrogenase
NCBI Official Symbol:SORD��
NCBI Official Synonym Symbols:SORD1; HEL-S-95n��
NCBI Protein Information:sorbitol dehydrogenase
UniProt Protein Name:Sorbitol dehydrogenase
UniProt Synonym Protein Names:L-iditol 2-dehydrogenase
Protein Family:Serine 3-dehydrogenase
UniProt Gene Name:SORD��
UniProt Entry Name:DHSO_HUMAN

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