The Human SPARCL1 (SPARC-like protein 1) ELISA Kit is specifically designed for the accurate quantification of SPARCL1 levels in human biological samples including serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit guarantees reliable and reproducible results, making it an invaluable tool for various research applications.SPARCL1, also known as Hevin, is a matricellular protein that plays a critical role in tissue remodeling, cell adhesion, and regulation of cell migration. It is involved in various biological processes such as wound healing, inflammation, and tumor progression, making it a potential biomarker for studying these processes and developing therapeutic interventions.
With the Human SPARCL1 ELISA Kit, researchers can accurately quantify SPARCL1 levels in human samples, allowing for a better understanding of its role in health and disease. The kit provides a simple and efficient method for analyzing SPARCL1 levels, making it a valuable tool for researchers in the fields of cancer biology, tissue engineering, and regenerative medicine.
Product Name:
Human SPARC Like Protein 1 (SPARCL1) ELISA Kit
Product Code:
HUDL02750
Size:
96 Assays
Target:
SPARCL1
Synonyms:
SC1, PIG33, Mast9, Hevin, High endothelial venule protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
