Sperm Equatorial Segment Protein 1 (SPESP1) is a member of the SPESP1 family. SPESP1 is highly expressed in the testis, where it is localized to the acrosome of postmeiotic stages of spermiogenesis; it is expressed at lower levels in the placenta and fetal lung. SPESP1 is involved in the multicellular organisimal development. Disruption of SPESP1 leads to abnormal distribution of sperm proteins resulting in a detached membrane from the equatorial segment and less fertile sperm. SPESP1 may interact with IZUMO1 and MN9 antigen and it contains an N-glycosylation site as well as several cAMP-dependent kinase, protein kinase C, and casein kinase II consensus phosphorylation sites.
Product Name:
Human SPESP1 Recombinant Protein (RPES0141)
Product Code:
RPES0141
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Sperm Equatorial Segment Protein 1, ESP, Equatorial Segment Protein, SP-ESP, Glycosylated 38 kDa Sperm Protein C-7/8, SPESP1
Accession:
Q6UW49
Sequence:
Tyr20-Tyr350
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Sperm Equatorial Segment Protein 1 is produced by our Mammalian expression system and the target gene encoding Tyr20-Tyr350 is expressed with a 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
37.9 kDa
AP Mol Mass:
58 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB,150mM NaCl,pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
