The Human sSele (Soluble E-selectin) ELISA Kit is specifically designed for the precise measurement of soluble E-selectin levels in human serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.E-selectin is a key adhesion molecule involved in inflammation and immune response, playing a critical role in the recruitment of leukocytes to sites of infection or injury. Elevated levels of soluble E-selectin have been linked to various inflammatory conditions, including cardiovascular diseases, autoimmune disorders, and diabetes, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.Overall, the Human sSele (Soluble E-selectin) ELISA Kit offers reliable and reproducible results, making it an essential tool for researchers investigating the role of E-selectin in health and disease.
Product Name:
Human sSELE (Soluble E-Selectin) ELISA Kit
SKU:
HUES02004
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
