Human TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit
The Human TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit is expertly designed for the precise quantification of Tissue Factor Pathway Inhibitor (TFPI) levels in a variety of biological samples. TFPI is a crucial anticoagulant protein that regulates blood coagulation and inhibits clot formation by modulating the tissue factor pathway. Its role in hemostasis and thrombosis underscores its significance in maintaining vascular integrity and preventing excessive blood clot formation. Accurate measurement of TFPI is essential for unraveling its contribution to coagulation disorders, cardiovascular diseases, and other pathophysiological conditions. Our TFPI ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under strict quality control standards, this kit delivers reliable performance and user-friendly operation, making it an excellent choice for cutting-edge research applications. Trust Assay Genie's TFPI ELISA Kit for dependable quantification of this critical anticoagulant protein in your scientific investigations.
Product Name:
Human TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit
SKU:
AEES00166
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
