The Human TSPAN30 (CD63) Tetraspanin 30 CLIA Kit is a highly accurate and reliable assay designed for the quantitative measurement of TSPAN30 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, allowing for precise and reproducible results for a variety of research applications.Tetraspanin 30, also known as CD63, is a key protein involved in cell adhesion, migration, and signaling. It plays a critical role in various cellular processes, including cell proliferation, immune response, and cancer metastasis.
Elevated levels of TSPAN30 have been associated with various diseases, making it a valuable biomarker for studying disease progression and potential therapeutic interventions.With the Human TSPAN30 (CD63) Tetraspanin 30 CLIA Kit, researchers can confidently measure TSPAN30 levels in human samples, helping to advance our understanding of its role in disease pathogenesis and ultimately leading to the development of novel diagnostic and treatment strategies.
Product Name:
Human TSPAN30/CD63 (Tetraspanin 30) CLIA Kit
SKU:
HUES01268
Target:
Human TSPAN30/CD63 (Tetraspanin 30)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TSPAN30/CD63. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TSPAN30/CD63 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TSPAN30/CD63, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TSPAN30/CD63. You can calculate the concentration of Human TSPAN30/CD63 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
92-106
94-108
Average (%)
107
97
102
1:4
Range (%)
91-104
97-110
93-106
Average (%)
97
103
98
1:8
Range (%)
93-106
99-112
94-110
Average (%)
101
105
102
1:16
Range (%)
100-117
86-99
91-103
Average (%)
107
92
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
98
EDTA plasma (n=5)
94-106
100
Cell culture media (n=5)
96-112
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
107.29
307.39
952.74
110.99
285.01
1012.31
Standard deviation
11.34
30.68
70.03
10.68
33.35
83.21
C V (%)
10.57
9.98
7.35
9.62
11.7
8.22
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TSPAN30/CD63 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TSPAN30/CD63 in samples. No significant cross-reactivity or interference between Human TSPAN30/CD63 and analogues was observed.
