Human UBE2D4 Recombinant Protein (RPPB2372)
- SKU:
- RPPB2372
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9Y2X8
- Research Area:
- Enzymes
Description
| Product Name: | Human UBE2D4 Recombinant Protein |
| Product Code: | RPPB2372 |
| Size: | 20µg |
| Species: | Human |
| Target: | UBE2D4 |
| Synonyms: | Ubiquitin-conjugating enzyme E2D 4 (putative), UBCH5D, HBUCE1, Ubiquitin carrier protein D4, Ubiquitin-protein ligase D4, EC 6.3.2.19. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The SRGN solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 0.1M NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MALKRIQKEL TDLQRDPPAQ CSAGPVGDDL FHWQATIMGP NDSPYQGGVF FLTIHFPTDY PFKPPKVAFT TKIYHPNINS NGSICLDILR SQWSPALTVS KVLLSICSLL CDPNPDDPLV PEIAHTYKAD REKYNRLARE WTQKYAM |
UBE2D4 is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzymes (E2 enzymes or ubiquitin-carrier enzymes) execute the second step in the ubiquitination reaction which marks a protein for degradation using the proteasome. UBE2D4 receives ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro UBE2D4 can promote polyubiquitination using all 7 ubiquitin Lys residues, but usually selects 'Lys-11' and 'Lys-48'-linked polyubiquitination.
UBE2D4 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 167 amino acids (1-147) and having a molecular mass of 18.8 kDa.UBE2D4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | UBE2D4: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family. |
| UniProt Protein Details: | Protein type:EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system; Ligase Chromosomal Location of Human Ortholog: 7p13 Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: protein ubiquitination |
| UniProt Code: | Q9Y2X8 |
| NCBI GenInfo Identifier: | 74753478 |
| NCBI Gene ID: | 51619 |
| NCBI Accession: | Q9Y2X8.1 |
| UniProt Secondary Accession: | Q9Y2X8,A4D1V0, |
| UniProt Related Accession: | Q9Y2X8 |
| Molecular Weight: | 16,649 Da |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 D4 |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 D4 (putative) |
| NCBI Official Symbol: | UBE2D4�� |
| NCBI Official Synonym Symbols: | HBUCE1�� |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 D4 |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 D4 |
| UniProt Synonym Protein Names: | E2 ubiquitin-conjugating enzyme D4; HBUCE1; Ubiquitin carrier protein D4; Ubiquitin-protein ligase D4 |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2D4�� |
| UniProt Entry Name: | UB2D4_HUMAN |
