Human UBE2E3 Recombinant Protein (RPPB2374)
- SKU:
- RPPB2374
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q969T4
- Research Area:
- Enzymes
Description
| Product Name: | Human UBE2E3 Recombinant Protein |
| Product Code: | RPPB2374 |
| Size: | 20µg |
| Species: | Human |
| Target: | UBE2E3 |
| Synonyms: | UBCH9, UbcM2, UBE2E3, Ubiquitin-Conjugating Enzyme E2E 3, UBCH9, Ubiquitin-Conjugating Enzyme E2E 3 (Homologous To Yeast UBC4/5), Ubiquitin-Conjugating Enzyme E2E 3 (UBC4/5 Homolog, Yeast), Ubiquitin-Conjugating Enzyme E2-23 KDa, Ubiquitin Carrier Protein E3, Ubiquitin-Protein Ligase E3, EC 6.3.2.19, Ubiquitin-Conjugating Enzyme E2 E3, UBCE4. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | UBE2E3 protein solution (1mg/ml) containing Phosphate buffered saline (pH7.4), 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 80.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMSSDRQR SDDESPSTSS GSSDADQRDP AAPEPEEQEE RKPSATQQKK NTKLSSKTTA KLSTSAKRIQ KELAEITLDP PPNCSAGPKG DNIYEWRSTI LGPPGSVYEG GVFFLDITFS SDYPFKPPKV TFRTRIYHCN INSQGVICLD ILKDNWSPAL TISKVLLSIC SLLTDCNPAD PLVGSIATQY LTNRAEHDRI ARQWTKRYAT |
UBE2E3 or Ubiquitin-conjugating enzyme E2 E3 is part of the E2 ubiquitinconjugating enzyme family. UBE2E3 is needed for the destruction of mitotic cyclins and for cell cycle progression. The ubiquitination process covalently attaches to a short protein of 76 amino acids called ubiquitin, to a lysine residue on the target protein. When a protein has been tagged with one ubiquitin molecule, other rounds of ubiquitination form a polyubiquitin chain that is recognized by the proteasome's 19S regulatory particle, triggering the ATP-dependent unfolding of the target protein which grants passage into the proteasome's 20S core particle, where proteases degrade the target into short peptide fragments for recycling by the cell.
UBE2E3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 230 amino acids (1-207 a.a) and having a molecular mass of 25.3kDa.UBE2E3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | UBE2E3: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest. Belongs to the ubiquitin-conjugating enzyme family. |
| UniProt Protein Details: | Protein type:EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system; Ligase Chromosomal Location of Human Ortholog: 2q32.1 Molecular Function:protein binding; ubiquitin-protein ligase activity |
| NCBI Summary: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2013] |
| UniProt Code: | Q969T4 |
| NCBI GenInfo Identifier: | 47606197 |
| NCBI Gene ID: | 10477 |
| NCBI Accession: | Q969T4.1 |
| UniProt Secondary Accession: | Q969T4,Q5U0R7, Q7Z4W4, B2RAD6, D3DPG3, |
| UniProt Related Accession: | Q969T4 |
| Molecular Weight: | 22,913 Da |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 E3 |
| NCBI Synonym Full Names: | ubiquitin conjugating enzyme E2 E3 |
| NCBI Official Symbol: | UBE2E3�� |
| NCBI Official Synonym Symbols: | UBCH9; UbcM2�� |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 E3 |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 E3 |
| UniProt Synonym Protein Names: | E2 ubiquitin-conjugating enzyme E3; UbcH9; Ubiquitin carrier protein E3; Ubiquitin-conjugating enzyme E2-23 kDa; Ubiquitin-protein ligase E3 |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2E3�� |
| UniProt Entry Name: | UB2E3_HUMAN |
