Human UBE2H Recombinant Protein (RPPB2378)
- SKU:
- RPPB2378
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P62256
- Research Area:
- Enzymes
Description
| Product Name: | Human UBE2H Recombinant Protein |
| Product Code: | RPPB2378 |
| Size: | 20µg |
| Species: | Human |
| Target: | UBE2H |
| Synonyms: | Ubiquitin-conjugating enzyme E2 H, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H, UBE2H, GID3, UBC8, UBCH. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The UBE2H solution (1mg/ml) contains 20mM Tris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT and 50mM NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMSSPSPG KRRMDTDVVK LIESKHEVTI LGGLNEFVVK FYGPQGTPYE GGVWKVRVDL PDKYPFKSPS IGFMNKIFHP NIDEASGTVC LDVINQTWTA LYDLTNIFES FLPQLLAYPN PIDPLNGDAA AMYLHRPEEY KQKIKEYIQK YATEEALKEQ EEGTGDSSSE SSMSDFSEDE AQDMEL |
Ubiquitin-conjugating enzyme E2 H (UBE2H) is a member of the ubiquitin-conjugating enzyme family. Protein modification with ubiquitin is a vital cellular apparatus for directing abnormal or short-lived proteins for degradation. Ubiquitination requires at least 3 classes of enzymes: ubiquitin-activating enzymes (E1s) ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2H receives ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. UBE2H protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs.
UBE2H Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 206 amino acids (1-183) and having a molecular mass of 23.1kDa.UBE2H is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | UBE2H: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A. Belongs to the ubiquitin-conjugating enzyme family. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; EC 6.3.2.19; Mitochondrial; Ubiquitin ligase; Ligase Chromosomal Location of Human Ortholog: 7q32 Cellular Component: cytoplasm Molecular Function:protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity Biological Process: ubiquitin-dependent protein catabolic process |
| NCBI Summary: | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011] |
| UniProt Code: | P62256 |
| NCBI GenInfo Identifier: | 51338683 |
| NCBI Gene ID: | 7328 |
| NCBI Accession: | P62256.1 |
| UniProt Secondary Accession: | P62256,P37286, Q7Z6F4, A4D1L6, C9JY93, |
| UniProt Related Accession: | P62256 |
| Molecular Weight: | 183 |
| NCBI Full Name: | Ubiquitin-conjugating enzyme E2 H |
| NCBI Synonym Full Names: | ubiquitin-conjugating enzyme E2H |
| NCBI Official Symbol: | UBE2H�� |
| NCBI Official Synonym Symbols: | GID3; UBC8; UBCH; UBCH2; E2-20K�� |
| NCBI Protein Information: | ubiquitin-conjugating enzyme E2 H; ubiquitin-protein ligase H; ubiquitin carrier protein H; GID complex subunit 3, UBC8 homolog; ubiquitin-conjugating enzyme E2-20K; ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast); ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8) |
| UniProt Protein Name: | Ubiquitin-conjugating enzyme E2 H |
| UniProt Synonym Protein Names: | UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H |
| Protein Family: | Ubiquitin-conjugating enzyme |
| UniProt Gene Name: | UBE2H�� |
| UniProt Entry Name: | UBE2H_HUMAN |
