Anti-IDH2 Antibody (CAB7190)

Antibody Name:	Anti-IDH2 Antibody
Antibody SKU:	CAB7190
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP ChIP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 193-452 of human IDH2 (NP_002159.2).

Application:	WB IHC IF IP ChIP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50 IP 1:50 - 1:200 ChIP 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HL-60, MCF-7, Jurkat, HepG2, U-87MG, Mouse kidney, Mouse heart, Rat lung, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 193-452 of human IDH2 (NP_002159.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KMVF TPKD GSGV KEWE VYNF PAGG VGMG MYNT DESI SGFA HSCF QYAI QKKW PLYM STKN TILK AYDG RFKD IFQE IFDK HYKT DFDK NKIW YEHR LIDD MVAQ VLKS SGGF VWAC KNYD GDVQ SDIL AQGF GSLG LMTS VLVC PDGK TIEA EAAH GTVT RHYR EHQK GRPT STNP IASI FAWT RGLE HRGK LDGN QDLI RFAQ MLEK VCVE TVES GAMT KDLA GCIH GLSN VKLN EHFL NTTD FLDT IKSN LDRA LGRQ
Gene ID:	3418
Uniprot:	P48735
Cellular Location:	Mitochondrion
Calculated MW:	45kDa/50kDa
Observed MW:	51kDa

Synonyms:

IDH2, D2HGA2, ICD-M, IDH, IDHM, IDP, IDPM, mNADP-IDH

Background:

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	IDH2: a mitochondrial oxidoreductase that catalyzes the third step of the citric acid cycle: the oxidative decarboxylation of isocitrate, consuming NADP(+), and producing alpha-ketoglutarate (alpha-KG) and CO2. Can also use oxalosuccinate as a substrate. Alpha-KG is an activator the dioxygenases that hydroxylate the transcription factor HIF and lead to its degradation by VHL. Since HIF turns on oncogenic pathways, IDH2 has apparent tumor suppressor activity. Homo-dimerization is required for activity. Each subunit binds 1 magnesium or manganese ion. May tightly associate or interact with the pyruvate dehydrogenase complex. A somatic mutation resulting in the R172K conversion results in a neomorphic enzyme activity that converts isocitrate to R(-)-2-hydroxyglutarate (2HG) occurs in a significant percentage of patients with cytogenetically normal acute myeloid leukemia (AML). Elevated levels of 2HG are also correlated with an elevated risk of malignant brain tumors.
UniProt Protein Details:	Protein type:Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.42; Other Amino Acids Metabolism - glutathione; Oxidoreductase Chromosomal Location of Human Ortholog: 15q26.1 Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane Molecular Function:magnesium ion binding; isocitrate dehydrogenase (NADP+) activity; NAD binding Biological Process: glyoxylate cycle; isocitrate metabolic process; cellular metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; 2-oxoglutarate metabolic process Disease: D-2-hydroxyglutaric Aciduria 2
NCBI Summary:	Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
UniProt Code:	P48735
NCBI GenInfo Identifier:	20141568
NCBI Gene ID:	3418
NCBI Accession:	P48735.2
UniProt Secondary Accession:	P48735,Q96GT3, B2R6L6, B4DFL2,
UniProt Related Accession:	P48735
Molecular Weight:	452
NCBI Full Name:	Isocitrate dehydrogenase
NCBI Synonym Full Names:	isocitrate dehydrogenase 2 (NADP+), mitochondrial
NCBI Official Symbol:	IDH2
NCBI Official Synonym Symbols:	IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
NCBI Protein Information:	isocitrate dehydrogenase [NADP], mitochondrial; isocitrate dehydrogenase [NADP], mitochondrial; NADP(+)-specific ICDH; oxalosuccinate decarboxylase
UniProt Protein Name:	Isocitrate dehydrogenase [NADP], mitochondrial
UniProt Synonym Protein Names:	ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase
Protein Family:	Isocitrate dehydrogenase
UniProt Gene Name:	IDH2
UniProt Entry Name:	IDHP_HUMAN

	Western blot analysis of extracts of various cell lines, using IDH2 antibody (CAB7190) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using IDH2 antibody (CAB7190) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using IDH2 antibody (CAB7190) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using IDH2 antibody (CAB7190) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug IDH2 antibody (CAB7190). Western blot was performed from the immunoprecipitate using IDH2 antibody (CAB7190) at a dilition of 1:1000.

Antibodies	ELISA Kits
Anti-IDH2 Antibody (CAB11270)	Human IDH2 / Isocitrate dehydrogenase 2 ELISA Kit
IDH2 Antibody (PACO09864)	Human Isocitrate dehydrogenase [NADP], mitochondrial (IDH2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)