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Anti-IGF1R Antibody (CAB12736)

SKU:
CAB12736
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
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Description

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Antibody Name:Anti-IGF1R Antibody
Antibody SKU:CAB12736
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide of human IGF1R
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Mouse
Positive Samples:Mouse kidney, Mouse lung, Mouse testis
Immunogen:A synthetic peptide of human IGF1R
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:3480
Uniprot:P08069
Cellular Location:Cell membrane, Single-pass type I membrane protein
Calculated MW:154kDa
Observed MW:100kDa
Synonyms:IGF1R, CD221, IGFIR, IGFR, JTK13
Background:This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
UniProt Protein Function:IGF1R: a receptor tyrosine kinase that binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. Functions as an anti-apoptotic agent by enhancing cell survival. Can play a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Mutated in rare cases of pre- and post-natal growth retardation. One SNP associated with increased human longevity. Increased expression of IGF1R and other pathway members associated with progression and malignancy in a range of cancers. Inhibitors: AG1024, AEW541.
UniProt Protein Details:

Protein type:Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; Membrane protein, integral; EC 2.7.10.1; TK group; InsR family

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: integral to plasma membrane; intracellular membrane-bound organelle; membrane; plasma membrane; receptor complex

Molecular Function:ATP binding; identical protein binding; insulin binding; insulin receptor binding; insulin receptor substrate binding; insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor receptor activity; phosphoinositide 3-kinase binding; protein binding; protein-tyrosine kinase activity

Biological Process: brain development; epidermis development; exocrine pancreas development; immune response; inactivation of MAPKK activity; insulin receptor signaling pathway; insulin-like growth factor receptor signaling pathway; male sex determination; mammary gland development; negative regulation of apoptosis; negative regulation of protein kinase B signaling cascade; negative regulation of transcription factor activity; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of MAPKKK cascade; positive regulation of mitosis; positive regulation of protein kinase B signaling cascade; protein amino acid autophosphorylation; protein tetramerization; regulation of JNK cascade; signal transduction

Disease: Insulin-like Growth Factor I, Resistance To

NCBI Summary:This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Code:P08069
NCBI GenInfo Identifier:124240
NCBI Gene ID:3480
NCBI Accession:P08069.1
UniProt Secondary Accession:P08069,Q14CV2, Q9UCC0, B1B5Y2,
UniProt Related Accession:P08069
Molecular Weight:
NCBI Full Name:Insulin-like growth factor 1 receptor
NCBI Synonym Full Names:insulin like growth factor 1 receptor
NCBI Official Symbol:IGF1R  
NCBI Official Synonym Symbols:IGFR; CD221; IGFIR; JTK13  
NCBI Protein Information:insulin-like growth factor 1 receptor
UniProt Protein Name:Insulin-like growth factor 1 receptor
UniProt Synonym Protein Names:Insulin-like growth factor I receptor; IGF-I receptor
Protein Family:Insulin-like growth factor 1 receptor
UniProt Gene Name:IGF1R  
UniProt Entry Name:IGF1R_HUMAN
Anti-IGF1R Antibody (CAB12736)Western blot analysis of extracts of various cell lines, using IGF1R antibody (CAB12736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

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