Western blot analysis of extracts of various cell lines, using INSR/IGF1R antibody (CAB21984) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The INSR/IGF1R Monoclonal Antibody (CAB21984) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed for use in Western blot applications, specifically targets the INSR/IGF1R protein, allowing for accurate detection and analysis in different samples.The functionality of INSR and IGF1R receptors is essential for the regulation of various cellular functions, including growth, metabolism, and differentiation. Dysregulation of these receptors has been linked to diseases such as diabetes, cancer, and metabolic disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
INSR/IGF1R Monoclonal Antibody
SKU:
CAB21984
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC54074
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Axon, Caveola, Dendrite Membrane, External Side Of Plasma Membrane, Extracellular Exosome, Late Endosome, Lysosome, Plasma Membrane.
Calculated MW:
156kDa
Observed MW:
97kDa
This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
3643 3480
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using INSR/IGF1R Rabbit mAb (CAB21984) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from 3T3L1 cells, using INSR/IGF1R Rabbit mAb (CAB21984) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
