null

Anti-IRAK1 Antibody (CAB12624)

SKU:
CAB12624
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
  • Immunology Antibodies 1 Anti-IRAK1 Antibody CAB12624
  • Immunology Antibodies 1 Anti-IRAK1 Antibody CAB12624
  • Immunology Antibodies 1 Anti-IRAK1 Antibody CAB12624
  • Immunology Antibodies 1 Anti-IRAK1 Antibody CAB12624
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-IRAK1 Antibody
Antibody SKU:CAB12624
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 424-633 of human IRAK1 (NP_001020414.1).
Application:WB IF
Recommended Dilution:WB 1:1000 - 1:3000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, A-549, Mouse liver, Mouse kidney, Mouse skeletal muscle, Rat liver, Rat kidney, Rat skeletal muscle
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 424-633 of human IRAK1 (NP_001020414.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VKTH GART KYLV YERL EKLQ AVVA GVPG HSEA ASCI PPSP QENS YVSS TGRA HSGA APWQ PLAA PSGA SAQA AEQL QRGP NQPV ESDE SLGG LSAA LRSW HLTP SCPL DPAP LREA GCPQ GDTA GESS WGSG PGSR PTAV EGLA LGSS ASSS SEPP QIII NPAR QKMV QKLA LYED GALD SLQL LSSS SLPG LGLE QDRQ GPEE SDEF QS
Gene ID:3654
Uniprot:P51617
Cellular Location:Cytoplasm, Lipid droplet, Nucleus
Calculated MW:68kDa/73kDa/74kDa/76kDa
Observed MW:90-100kDa
Synonyms:IRAK1, IRAK, pelle
Background:This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:IRAK1: a TKL kinase of the IRAK family. Involved in Toll/IL-1 signaling. One of two putative serine/threonine kinases that become associated with the interleukin-1 receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Extensively phosphorylated after its association with IL1-R-1. Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Three isoforms of the human protein and produced by alternative splicing. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
UniProt Protein Details:

Protein type:Protein kinase, TKL; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; IRAK family

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cytoplasm; cytosol; endosome membrane; lipid particle; nucleus; plasma membrane

Molecular Function:kinase activity; NF-kappaB-inducing kinase activity; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase activity; protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; activation of NF-kappaB transcription factor; activation of NF-kappaB-inducing kinase; inhibition of NF-kappaB transcription factor; JNK cascade; lipopolysaccharide-mediated signaling pathway; MyD88-dependent toll-like receptor signaling pathway; negative regulation of apoptosis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of MAP kinase activity; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein oligomerization; regulation of cytokine and chemokine mediated signaling pathway; response to lipopolysaccharide; toll-like receptor 2 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway

NCBI Summary:This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P51617
NCBI GenInfo Identifier:8928535
NCBI Gene ID:3654
NCBI Accession:P51617.2
UniProt Secondary Accession:P51617,Q7Z5V4, Q96C06, Q96RL2, D3DWW3, D3DWW4,
UniProt Related Accession:P51617
Molecular Weight:68,022 Da
NCBI Full Name:Interleukin-1 receptor-associated kinase 1
NCBI Synonym Full Names:interleukin 1 receptor associated kinase 1
NCBI Official Symbol:IRAK1  
NCBI Official Synonym Symbols:IRAK; pelle  
NCBI Protein Information:interleukin-1 receptor-associated kinase 1
UniProt Protein Name:Interleukin-1 receptor-associated kinase 1
Protein Family:Interleukin-1 receptor-associated kinase
UniProt Gene Name:IRAK1  
UniProt Entry Name:IRAK1_HUMAN
Anti-IRAK1 Antibody (CAB12624)Western blot analysis of extracts of various cell lines, using IRAK1 antibody (CAB12624) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-IRAK1 Antibody (CAB12624)Immunofluorescence analysis of C6 cells using IRAK1 antibody (CAB12624) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-IRAK1 Antibody (CAB12624)Immunofluorescence analysis of HeLa cells using IRAK1 antibody (CAB12624) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-IRAK1 Antibody (CAB12624)Immunofluorescence analysis of NIH/3T3 cells using IRAK1 antibody (CAB12624) at dilution of 1:100. Blue: DAPI for nuclear staining.

Related Products