Anti-KDM4A Antibody (CAB7953)

Antibody Name:	KDM4A Rabbit Polyclonal Antibody
Antibody SKU:	CAB7953
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 585-815 of human KDM4A (NP_055478.2).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	BT-474, THP-1, Mouse thymus

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 585-815 of human KDM4A (NP_055478.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FSLE ENKK SKGR RQPL SKLP RHHP LVLQ ECVS DDET SEQL TPEE EAEE TEAW AKPL SQLW QNRP PNFE AEKE FNET MAQQ APHC AVCM IFQT YHQV EFGG FNQN CGNA SDLA PQKQ RTKP LIPE MCFT STGC STDI NLST PYLE EDGT SILV SCKK CSVR VHAS CYGV PPAK ASED WMCS RCSA NALE EDCC LCSL RGGA LQRA NDDR WVHV SCAV AILE ARFV NIAE RSP
Gene ID:	9682
Uniprot:	O75164
Cellular Location:	Nucleus
Calculated MW:	54kDa/120kDa
Observed MW:	150kDa

Synonyms:	KDM4A, JHDM3A, JMJD2, JMJD2A, TDRD14A
Background:	This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.

UniProt Protein Function:	JMJD2A: Histone demethylase that specifically demethylates 'Lys- 9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. Interacts with histone deacetylase proteins HDAC1, HDAC2 and HDAC3. Interacts with RB and NCOR1. Interacts with HTLV-1 Tax protein. Ubiquitous. Belongs to the JHDM3 histone demethylase family.
UniProt Protein Details:	Protein type:EC 1.14.11.-; Demethylase; Oxidoreductase; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 1p34.1 Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus Molecular Function:histone demethylase activity; histone demethylase activity (H3-K36 specific); methylated histone residue binding; protein binding; ubiquitin protein ligase binding Biological Process: histone demethylation; negative regulation of autophagy; negative regulation of transcription, DNA-dependent
NCBI Summary:	This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq, Apr 2009]
UniProt Code:	O75164
NCBI GenInfo Identifier:	308153457
NCBI Gene ID:	9682
NCBI Accession:	O75164.2
UniProt Secondary Accession:	O75164,Q5VVB1,
UniProt Related Accession:	O75164
Molecular Weight:	54,697 Da
NCBI Full Name:	Lysine-specific demethylase 4A
NCBI Synonym Full Names:	lysine demethylase 4A
NCBI Official Symbol:	KDM4A
NCBI Official Synonym Symbols:	JMJD2; JHDM3A; JMJD2A; TDRD14A
NCBI Protein Information:	lysine-specific demethylase 4A
UniProt Protein Name:	Lysine-specific demethylase 4A
UniProt Synonym Protein Names:	JmjC domain-containing histone demethylation protein 3A; Jumonji domain-containing protein 2A
Protein Family:	Lysine-specific demethylase
UniProt Gene Name:	KDM4A
UniProt Entry Name:	KDM4A_HUMAN

	Western blot analysis of extracts of various cell lines, using KDM4A antibody (CAB7953) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse lung using KDM4A antibody (CAB7953) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse liver using KDM4A antibody (CAB7953) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using KDM4A antibody (CAB7953) at dilution of 1:100 (40x lens).

Antibodies

Anti-KDM4A Antibody (CAB12551)

Anti-KDM4A Antibody (CAB9267)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)