[KO Validated] active + pro Caspase-3 Monoclonal Antibody (CAB19654)
The [KO Validated] active + pro Caspase-3 Monoclonal Antibody (CAB19654) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbit monoclonal technology, is highly specific and sensitive for detecting active pro-caspase-3 in various biological samples.Validated for knockout (KO) studies, this antibody is ideal for confirming the presence and activity of pro-caspase-3 in cell lysates or tissue samples. Its high specificity ensures accurate results in Western blot applications, making it an essential tool for researchers investigating apoptosis, cell signaling pathways, and potential therapeutic targets in cancer research and other disease contexts.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
[KO Validated] active + pro Caspase-3 Monoclonal Antibody
SKU:
CAB19654
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC0133
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CPP32, SCA-1, CPP32B, -3
Positive Sample:
293T, Jurkat treated with Etoposide, L929 treated with staurosporine, NIH/3T3 treated with staurosporine
Cellular Localization:
Cytoplasm.
Calculated MW:
32kDa
Observed MW:
17kDa/32kDa
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Purification Method
Affinity purification
Gene ID
836
RRID
AB_2862718
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using [KO Validated] Caspase-3 Rabbit mAb (CAB19654) at 1:1000 dilution. Jurkat cells were treated with Etoposide (25 uM) at 37℃ for 5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from L929 cells using [KO Validated] Caspase-3 Rabbit mAb (CAB19654) at 1:1000 dilution. L929 cells were treated with staurosporine(1 μM) for 3 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from wild type (WT) and Caspase-3 knockout (KO) 293T cells using [KO Validated] Caspase-3 Rabbit mAb (CAB19654) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
