Anti-active + pro Caspase-3 Antibody (CAB19654)

Antibody Name:	Anti-active + pro Caspase-3 Antibody [KO Validated]
Antibody SKU:	CAB19654
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human active + pro Caspase-3.

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, Mouse lung, Mouse liver, NIH/3T3, Rat spleen, Rat liver, 293T

Immunogen:	A synthesized peptide derived from human active + pro Caspase-3.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	836
Uniprot:	P42574
Cellular Location:	Cytoplasm
Calculated MW:	17kDa/32kDa
Observed MW:	17KDa/32kDa/35KDa

	Western blot analysis of extracts from normal (control) and active + pro Caspase-3 knockout (KO) 293T cells, using active + pro Caspase-3 antibody (CAB19654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts of various cell lines, using active + pro Caspase-3 antibody (CAB19654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts of various cell lines, using active + pro Caspase-3 antibody (CAB19654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
	Western blot analysis of extracts of Jurkat cells, using active + pro Caspase 3 antibody (CAB19654) at 1:1000 dilution.Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours . Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of HeLa cells using active + pro Caspase-3 antibody (CAB19654).HeLa cells were treated by Etoposide (25 uM) at 37'C for 5 hours. Blue: DAPI for nuclear staining.

Synonyms:

CPP32, CPP32B, SCA-1, Active Caspase 3, CASP3, active Caspase-3, Caspase 3, Caspase-3 p12, caspase-3

Background:

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

UniProt Protein Function:	CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-\|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.
UniProt Protein Details:	Protein type:Protease; EC 3.4.22.56; Apoptosis; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 4q34 Cellular Component: nucleoplasm; plasma membrane; nucleus; cytosol Molecular Function:peptidase activity; cyclin-dependent protein kinase inhibitor activity; protein binding; cysteine-type endopeptidase activity; aspartic-type endopeptidase activity Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; heart development; negative regulation of activated T cell proliferation; negative regulation of B cell proliferation; regulation of caspase activity; proteolysis; neuron differentiation; extracellular matrix disassembly; sensory perception of sound; B cell homeostasis; positive regulation of neuron apoptosis; response to wounding; erythrocyte differentiation; T cell homeostasis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis; response to UV; release of cytochrome c from mitochondria; cell fate commitment; negative regulation of cyclin-dependent protein kinase activity; keratinocyte differentiation; neuron apoptosis; induction of apoptosis via death domain receptors; caspase activation via cytochrome c; platelet formation; induction of apoptosis by oxidative stress; response to DNA damage stimulus; negative regulation of apoptosis
NCBI Summary:	This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]
UniProt Code:	P42574
NCBI GenInfo Identifier:	77416852
NCBI Gene ID:	836
NCBI Accession:	P42574.2
UniProt Secondary Accession:	P42574,Q96AN1, Q96KP2, A8K5M2, D3DP53,
UniProt Related Accession:	P42574
Molecular Weight:	277
NCBI Full Name:	Caspase-3
NCBI Synonym Full Names:	caspase 3, apoptosis-related cysteine peptidase
NCBI Official Symbol:	CASP3
NCBI Official Synonym Symbols:	CPP32; SCA-1; CPP32B
NCBI Protein Information:	caspase-3; CASP-3; CPP-32; apopain; procaspase3; protein Yama; PARP cleavage protease; cysteine protease CPP32; SREBP cleavage activity 1; caspase 3, apoptosis-related cysteine protease
UniProt Protein Name:	Caspase-3
UniProt Synonym Protein Names:	Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
Protein Family:	Caspase
UniProt Gene Name:	CASP3
UniProt Entry Name:	CASP3_HUMAN

Antibodies	ELISA Kits
Anti-Caspase-3 Antibody (CAB0214)	Human Caspase 3 ELISA Kit
Anti-Active Caspase-3 Antibody (CAB11021)	Human CASP2(Caspase-2 ) ELISA Kit
Anti-Caspase-3 Antibody (CAB11040)[KO Validated]	Human CASP3 (Caspase 3) CLIA Kit (HUES00017)
Anti-Caspase-3 Antibody (CAB11319)	Human CASP3 (Caspase 3) ELISA Kit (HUES01304)
Anti-Caspase-3 Antibody (CAB11953)[KO Validated]	Human Caspase-3 (CASP3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Mouse IgG (H+L) Antibody (CABS003)