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[KO Validated] Caspase-3 Rabbit Polyclonal Antibody (CAB16794)

SKU:
CAB16794
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
  • KO Validated Antibodies 1 Anti-Caspase-3 Antibody CAB16794KO Validated
  • KO Validated Antibodies 1 Anti-Caspase-3 Antibody CAB16794KO Validated
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] Caspase-3 Rabbit Polyclonal Antibody
SKU:CAB16794
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 28-127 of human Caspase-3 (NP_004337.2).
Sequence:DSGI SLDN SYKM DYPE MGLC IIIN NKNF HKST GMTS RSGT DVDA ANLR ETFR NLKY EVRN KNDL TREE IVEL MRDV SKED HSKR SSFV CVLL SHGE EGII
Tested Applications:WB IHC-P IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200
Synonyms:CPP32; SCA-1; CPP32B; -3
Positive Sample:293T,NIH/3T3,Jurkat
Cellular Localization:Cytoplasm.
Calculated MW:32kDa
Observed MW:32kDa/17kDa

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

Purification Method:Affinity purification
Gene ID:836
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] Caspase-3 Rabbit pAb (CAB16794) at 1:1000 dilution.Jurkat cells were treated by staurosporine(1 uM) for 3 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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