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[KO Validated] NF-kB p65/RelA Rabbit Monoclonal Antibody (CAB19653)

SKU:
CAB19653
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • KO Validated Antibodies 2 Anti-NF-kB p65 Antibody KO Validated CAB19653
  • KO Validated Antibodies 2 Anti-NF-kB p65 Antibody KO Validated CAB19653
  • KO Validated Antibodies 2 Anti-NF-kB p65 Antibody KO Validated CAB19653
  • KO Validated Antibodies 2 Anti-NF-kB p65 Antibody KO Validated CAB19653
  • KO Validated Antibodies 2 Anti-NF-kB p65 Antibody KO Validated CAB19653
€139 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:[KO Validated] NF-kB p65/RelA Rabbit Monoclonal Antibody
SKU:CAB19653
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat,Monkey
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 450-551 of human NF-κB p65 (Q04206).
Sequence:LGAL LGNS TDPA VFTD LASV DNSE FQQL LNQG IPVA PHTT EPML MEYP EAIT RLVT GAQR PPDP APAP LGAP GLPN GLLS GDED FSSI ADMD FSAL LSQI SS
Tested Applications:WB IHC-P IF/ICC ChIP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:500 - 1:1000 IF/ICC,1:50 - 1:200 ChIP,5μg antibody for 10μg-15μg of Chromatin
Synonyms:p65; CMCU; NFKB3; AIF3BL3; NF-kB p65/RelA
Positive Sample:PC-12, Mouse spleen, Mouse lung,HeLa,PC-12,COS-7,NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:60kDa
Observed MW:65kDa

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:5970
Clone Number:ARC51086
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using NF-kB p65/RelA Rabbit mAb (CAB19653) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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