Anti-PAK4 Antibody (CAB11646)

Antibody Name:	Anti-PAK4 Antibody
Antibody SKU:	CAB11646
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human PAK4 (NP_001014831.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	LNCaP, Mouse testis, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 120-280 of human PAK4 (NP_001014831.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EPAT TARG GPGK AGSR GRFA GHSE AGGG SGDR RRAG PEKR PKSS REGS GGPQ ESSR DKRP LSGP DVGT PQPA GLAS GAKL AAGR PFNT YPRA DTDH PSRG AQGE PHDV APNG PSAG GLAI PQSS SSSS RPPT RARG APSP GVLG PHAS EPQL APPA CTPA A
Gene ID:	10298
Uniprot:	O96013
Cellular Location:	Cytoplasm
Calculated MW:	47kDa/48kDa/54kDa/64kDa
Observed MW:	70KDa

Synonyms:

PAK4

Background:

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

UniProt Protein Function:	PAK4: a protein kinase of the STE20 family. A mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Interacts specifically with the GTP-bound form of Cdc42 and weakly activates the JNK family of MAP kinases. Overexpressed in several cancers. Required for ras-dependent anchorage-independent growth of tumor cell lines. Four alternatively spliced human isoforms have been reported.
UniProt Protein Details:	Protein type:Kinase, protein; EC 2.7.11.1; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKB subfamily Chromosomal Location of Human Ortholog: 19q13.2 Cellular Component: focal adhesion; Golgi apparatus Molecular Function:protein binding; receptor signaling protein serine/threonine kinase activity Biological Process: apoptosis; cell growth; cell migration; cell motility; cell proliferation; cytoskeleton organization and biogenesis; mitotic cell cycle; signal transduction
NCBI Summary:	PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O96013
NCBI GenInfo Identifier:	12585288
NCBI Gene ID:	10298
NCBI Accession:	O96013.1
UniProt Secondary Accession:	O96013,Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8, B4DGG6
UniProt Related Accession:	O96013
Molecular Weight:
NCBI Full Name:	Serine/threonine-protein kinase PAK 4
NCBI Synonym Full Names:	p21 (RAC1) activated kinase 4
NCBI Official Symbol:	PAK4
NCBI Protein Information:	serine/threonine-protein kinase PAK 4
UniProt Protein Name:	Serine/threonine-protein kinase PAK 4
UniProt Synonym Protein Names:	p21-activated kinase 4; PAK-4
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	PAK4
UniProt Entry Name:	PAK4_HUMAN

	Western blot analysis of extracts of LNCaP cells, using PAK4 antibody (CAB11646) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Western blot analysis of extracts of various cell lines, using PAK4 antibody (CAB11646) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

Anti-PAK4 Antibody (CAB2782)

Anti-PAK4 Antibody (CAB18059)[KO Validated]

Phospho-PAK4 (S474) Antibody (PACO03333)

PAK4 Antibody (PACO03334)

PAK4 Antibody (PACO11059)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)