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Anti-PPP2R1B Antibody (CAB18089)[KO Validated]

SKU:
CAB18089
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
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Description

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Antibody Name:Anti-PPP2R1B Antibody [KO Validated]
Antibody SKU:CAB18089
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 468-667 of human PPP2R1B (NP_859050.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 468-667 of human PPP2R1B (NP_859050.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YAIR EAAT NNLM KLVQ KFGT EWAQ NTIV PKVL VMAN DPNY LHRM TTLF CINA LSEA CGQE ITTK QMLP IVLK MAGD QVAN VRFN VAKS LQKI GPIL DTNA LQGE VKPV LQKL GQDE DMDV KYFA QEAI SVVA QRLR KLEF PVKD SGEP SVPR ADKN HFPR PTVP GEDM GKGP VYQL RGDT RDTL AQLG IAEL VHFS QSTD
Gene ID:5519
Uniprot:P30154
Cellular Location:
Calculated MW:52kDa/61kDa/66kDa/73kDa
Observed MW:66kDa
Anti-PPP2R1B Antibody [KO Validated] (CAB18089)Western blot analysis of extracts from normal (control) and PPP2R1B knockout (KO) 293T cells, using PPP2R1B antibody (CAB18089) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Synonyms:PPP2R1B, PP2A-Abeta, PR65B
Background:This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described.
UniProt Protein Function:PPP2R1B: The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Belongs to the phosphatase 2A regulatory subunit A family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 11q23.2

Cellular Component: lipid raft

Molecular Function:protein binding

Disease: Lung Cancer

NCBI Summary:This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes a beta isoform of the constant regulatory subunit A. Mutations in this gene have been associated with some lung and colon cancers. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
UniProt Code:P30154
NCBI GenInfo Identifier:116241236
NCBI Gene ID:5519
NCBI Accession:P30154.3
UniProt Secondary Accession:P30154,O75620, Q8NHV8, A8MY67, B0YJ69, B4DGQ6, B4DK91 B4DWW5, F8W8G1,
UniProt Related Accession:P30154
Molecular Weight:52,214 Da
NCBI Full Name:Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
NCBI Synonym Full Names:protein phosphatase 2 scaffold subunit Abeta
NCBI Official Symbol:PPP2R1B  
NCBI Official Synonym Symbols:PR65B; PP2A-Abeta  
NCBI Protein Information:serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
UniProt Protein Name:Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
UniProt Synonym Protein Names:PP2A subunit A isoform PR65-beta; PP2A subunit A isoform R1-beta
UniProt Gene Name:PPP2R1B  
UniProt Entry Name:2AAB_HUMAN

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