Anti-Smad1 Antibody (CAB19113)

Antibody Name:	Anti-Smad1 Antibody [KO Validated]
Antibody SKU:	CAB19113
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human Smad1

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	HUVEC, HeLa

Immunogen:	A synthesized peptide derived from human Smad1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4086
Uniprot:	Q15797
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	58kDa
Observed MW:	58kDa

	Western blot analysis of extracts from normal (control) and Smad1 knockout (KO) HeLa cells, using Smad1 antibody (CAB19113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts of HUVEC cells, using Smad1 antibody (CAB19113) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Synonyms:

BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1, Smad1, SMAD1

Background:

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

UniProt Protein Function:	SMAD1: transcription factor phosphorylated and activated by bone morphogenetic protein type 1 receptor kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated forms dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 4q31 Cellular Component: nucleoplasm; transcription factor complex; protein complex; cytoplasm; integral to membrane; nuclear inner membrane; intracellular; cytosol; nucleus Molecular Function:identical protein binding; protein binding; metal ion binding; receptor signaling protein activity; protein kinase binding; transcription factor activity; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity Biological Process: wound healing; cardiac muscle cell proliferation; primary microRNA processing; embryonic pattern specification; signal transduction; protein amino acid phosphorylation; mesodermal cell fate commitment; BMP signaling pathway; negative regulation of cell proliferation; homeostatic process; ureteric bud development; transforming growth factor beta receptor signaling pathway; midbrain development; inflammatory response; hindbrain development; response to drug; transcription, DNA-dependent; MAPKKK cascade; positive regulation of dendrite morphogenesis; response to organic nitrogen; SMAD protein complex assembly; positive regulation of osteoblast differentiation; gamete generation; cartilage development; positive regulation of transcription from RNA polymerase II promoter; osteoblast fate commitment
NCBI Summary:	The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15797
NCBI GenInfo Identifier:	13633915
NCBI Gene ID:	4086
NCBI Accession:	Q15797.1
UniProt Secondary Accession:	Q15797,Q16636, Q9UFT8, A8KAJ0, D3DNZ9,
UniProt Related Accession:	Q15797
Molecular Weight:	465
NCBI Full Name:	Mothers against decapentaplegic homolog 1
NCBI Synonym Full Names:	SMAD family member 1
NCBI Official Symbol:	SMAD1
NCBI Official Synonym Symbols:	BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
NCBI Protein Information:	mothers against decapentaplegic homolog 1; MAD homolog 1; Mad-related protein 1; TGF-beta signaling protein 1; mothers against DPP homolog 1; SMAD, mothers against DPP homolog 1; MAD, mothers against decapentaplegic homolog 1; transforming growth factor-beta signaling protein 1; transforming growth factor-beta-signaling protein 1
UniProt Protein Name:	Mothers against decapentaplegic homolog 1
UniProt Synonym Protein Names:	JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1; BSP-1
Protein Family:	Mothers against decapentaplegic
UniProt Gene Name:	SMAD1
UniProt Entry Name:	SMAD1_HUMAN

Antibodies	ELISA Kits
Anti-Smad1 Antibody (CAB1101)	Human Smad1 / MADH1 ELISA Kit
Anti-Phospho-SMAD1-S206 Antibody (CABP0295)	Human Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1) CLIA Kit (HUES00555)
Phospho-SMAD1 (S465) Antibody (PACO00293)	Human Smad1/MADH1 (Mothers Against Decapentaplegic Homolog 1) ELISA Kit (HUES02056)
SMAD1 Antibody (PACO01509)	Human Mothers against decapentaplegic homolog 1 (SMAD1) ELISA Kit
SMAD1 Antibody (PACO02035)	Smad1 (Phospho-Ser187) Transcription Factor Activity Assay

Secondary Antibody

Anti-HRP Goat Anti-Mouse IgG (H+L) Antibody (CABS003)