Anti-KPNA2 Antibody (CAB1623)

Antibody Name:	Anti-KPNA2 Antibody
Antibody SKU:	CAB1623
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human KPNA2 (NP_002257.1).

Application:	WB IP
Recommended Dilution:	WB 1:500 - 1:2000 IP 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	MCF7, PC-3, U-251MG, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human KPNA2 (NP_002257.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSTN ENAN TPAA RLHR FKNK GKDS TEMR RRRI EVNV ELRK AKKD DQML KRRN VSSF PDDA TSPL QENR NNQG TVNW SVDD IVKG INSS NVEN QLQA TQAA RKLL SREK QPPI DNII RAGL IPKF VSFL GRTD CSPI QFES AWAL TNIA SGTS EQTK AVVD GGAI PAFI SLLA SPHA HISE QAVW ALGN IAGD GSVF RDLV IKYG AVDP LLAL LAVP DMSS LACG YLRN LTWT LSNL CRNK NPAP PIDA VEQI LPTL VRLL HHDD PEVL ADTC WAIS YLTD GPNE RIGM VV
Gene ID:	3838
Uniprot:	P52292
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	57kDa
Observed MW:	55kDa

Synonyms:

KPNA2, IPOA1, QIP2, RCH1, SRP1-alpha, SRP1alpha

Background:

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	KPNA2: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Belongs to the importin alpha family.
UniProt Protein Details:	Protein type:Nuclear import; Karyopherin; Nucleoporin Chromosomal Location of Human Ortholog: 17q24.2 Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol Molecular Function:protein binding; histone deacetylase binding; nuclear localization sequence binding; protein transporter activity Biological Process: viral reproduction; cytokine and chemokine mediated signaling pathway; regulation of DNA recombination; NLS-bearing substrate import into nucleus; DNA metabolic process
NCBI Summary:	The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq, Jul 2008]
UniProt Code:	P52292
NCBI GenInfo Identifier:	1708480
NCBI Gene ID:	3838
NCBI Accession:	P52292.1
UniProt Secondary Accession:	P52292,Q53YE3, Q9BRU5, B9EJD6,
UniProt Related Accession:	P52292
Molecular Weight:	529
NCBI Full Name:	Importin subunit alpha-1
NCBI Synonym Full Names:	karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
NCBI Official Symbol:	KPNA2
NCBI Official Synonym Symbols:	QIP2; RCH1; IPOA1; SRP1alpha
NCBI Protein Information:	importin subunit alpha-1; pendulin; SRP1-alpha; RAG cohort 1; importin alpha 1; importin alpha 2; importin-alpha-P1; RAG cohort protein 1; importin subunit alpha-2; karyopherin subunit alpha-2
UniProt Protein Name:	Importin subunit alpha-1
UniProt Synonym Protein Names:	Karyopherin subunit alpha-2; RAG cohort protein 1; SRP1-alpha
UniProt Gene Name:	KPNA2
UniProt Entry Name:	IMA1_HUMAN

	Western blot analysis of extracts of various cell lines, using KPNA2 antibody (CAB1623) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunoprecipitation analysis of 200ug extracts of U-251MG cells, using 3 ug KPNA2 antibody (CAB1623). Western blot was performed from the immunoprecipitate using KPNA2 antibody (CAB1623) at a dilition of 1:1000.

Antibodies	ELISA Kits
Anti-KPNA2 Antibody (CAB5012)	Human KPNA2 / Karyopherin alpha 2 ELISA Kit
KPNA2 Antibody (PACO10152)	Human Importin subunit alpha-2 (KPNA2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)