Anti-LIG3 Antibody (CAB13507)

Antibody Name:	Anti-LIG3 Antibody
Antibody SKU:	CAB13507
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 730-1009 of human LIG3 (NP_039269.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, BT-474, SW480

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 730-1009 of human LIG3 (NP_039269.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AGGH DDAT LARL QNEL DMVK ISKD PSKI PSWL KVNK IYYP DFIV PDPK KAAV WEIT GAEF SKSE AHTA DGIS IRFP RCTR IRDD KDWK SATN LPQL KELY QLSK EKAD FTVV AGDE GSST TGGS SEEN KGPS GSAV SRKA PSKP SAST KKAE GKLS NSNS KDGN MQTA KPSA MKVG EKLA TKSS PVKV GEKR KAAD ETLC QTKV LLDI FTGV RLYL PPST PDFS RLRR YFVA FDGD LVQE FDMT SATH VLGS RDKN PAAQ QVSP EWIW ACIR KRRL VAPC
Gene ID:	3980
Uniprot:	P49916
Cellular Location:	Mitochondrion, Mitochondrion, Nucleus
Calculated MW:	95kDa/102kDa/106kDa/112kDa
Observed MW:	112kDa

Synonyms:

LIG3, LIG2

Background:

This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.

UniProt Protein Function:	LIG3: Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Belongs to the ATP-dependent DNA ligase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 6.5.1.1; Ligase Chromosomal Location of Human Ortholog: 17q11.2-q12 Cellular Component: nucleoplasm; mitochondrion; chromosome; synaptonemal complex; nucleus Molecular Function:protein binding; DNA binding; DNA ligase activity; zinc ion binding; DNA ligase (ATP) activity; ATP binding Biological Process: mitochondrial DNA repair; mitochondrion organization and biogenesis; negative regulation of DNA recombination; V(D)J recombination; base-excision repair, DNA ligation; DNA repair; cell cycle; lagging strand elongation; double-strand break repair via nonhomologous end joining; DNA ligation; nucleotide-excision repair; base-excision repair; cell division; double-strand break repair
NCBI Summary:	This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	P49916
NCBI GenInfo Identifier:	251757259
NCBI Gene ID:	3980
NCBI Accession:	P49916.2
UniProt Related Accession:	P49916
Molecular Weight:
NCBI Full Name:	DNA ligase 3
NCBI Synonym Full Names:	DNA ligase 3
NCBI Official Symbol:	LIG3
NCBI Official Synonym Symbols:	LIG2; LIG3alpha
NCBI Protein Information:	DNA ligase 3
UniProt Protein Name:	DNA ligase 3
UniProt Synonym Protein Names:	DNA ligase III; Polydeoxyribonucleotide synthase [ATP] 3
Protein Family:	DNA ligase
UniProt Gene Name:	LIG3
UniProt Entry Name:	DNLI3_HUMAN

	Western blot analysis of extracts of various cell lines, using LIG3 antibody (CAB13507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human colon using LIG3 Rabbit pAb (CAB13507) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-LIG3 Antibody (CAB1887)	Human LIG3 / DNA ligase 3 ELISA Kit
LIG3 Antibody (PACO06429)	Human DNA ligase 3 (LIG3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)