The LY75 Antibody (CAB10065) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the LY75 protein, this antibody enables the detection and analysis of LY75 expression in various cell types, making it a useful resource for studies in immunology and cancer research.LY75, also known as DEC-205, plays a crucial role in antigen uptake and presentation by dendritic cells, contributing to the regulation of immune responses.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
LY75 Antibody
SKU:
CAB10065
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD205, LY-75, CLEC13B, DEC-205, GP200-MR6, LY75
Positive Sample:
Daudi, U-937, THP-1, Mouse thymus
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
198kDa
Observed MW:
250kDa
Predicted to enable signaling receptor activity. Predicted to be involved in immune response and inflammatory response. Located in extracellular exosome.
Purification Method
Affinity purification
Gene ID
4065
RRID
AB_2757587
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using LY75 Rabbit pAb (CAB10065) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
