Anti-MAP2K4 Antibody (CAB7724)

Antibody Name:	Anti-MAP2K4 Antibody
Antibody SKU:	CAB7724
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human MAP2K4

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:1000 - 1:4000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, MCF7, SH-SY5Y, Rat skeletal muscle

Immunogen:	A synthetic peptide of human MAP2K4
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	6416
Uniprot:	P45985
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	44kDa/45kDa
Observed MW:	44kDa

Synonyms:

MAP2K4, JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1

Background:

This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	MKK4: dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38 but not ERK1 or -2. Mediates cellular responses to various cellular stresses and inflammatory cytokines. Phosphorylation by Akt inhibits MKK4 and suppresses stress-activated signal transduction.
UniProt Protein Details:	Protein type:Protein kinase, STE; Protein kinase, dual-specificity (non-receptor); Kinase, protein; EC 2.7.12.2; STE group; STE7 family Chromosomal Location of Human Ortholog: 17p12 Cellular Component: dendrite cytoplasm; axon; perikaryon; intracellular; cytosol; nucleus Molecular Function:protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; mitogen-activated protein kinase kinase kinase binding; JUN kinase kinase activity; ATP binding; protein kinase activity Biological Process: peptidyl-tyrosine phosphorylation; apoptosis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; activation of JNK activity; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of neuron apoptosis; toll-like receptor signaling pathway; JNK cascade; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; positive regulation of DNA replication; positive regulation of nitric-oxide synthase biosynthetic process
NCBI Summary:	This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:	P45985
NCBI GenInfo Identifier:	1170596
NCBI Gene ID:	6416
NCBI Accession:	P45985.1
UniProt Secondary Accession:	P45985,Q5U0B8, Q6FHX4, Q6P9H2, Q6PIE6, B2R7N7, B3KYB2 D3DTS5,
UniProt Related Accession:	P45985
Molecular Weight:	399
NCBI Full Name:	Dual specificity mitogen-activated protein kinase kinase 4
NCBI Synonym Full Names:	mitogen-activated protein kinase kinase 4
NCBI Official Symbol:	MAP2K4
NCBI Official Synonym Symbols:	JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
NCBI Protein Information:	dual specificity mitogen-activated protein kinase kinase 4; MEK 4; MAPKK 4; MAPK/ERK kinase 4; SAPK/ERK kinase 1; MAP kinase kinase 4; JNK-activated kinase 1; JNK-activating kinase 1; c-Jun N-terminal kinase kinase 1; stress-activated protein kinase kinase 1
UniProt Protein Name:	Dual specificity mitogen-activated protein kinase kinase 4
UniProt Synonym Protein Names:	JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated protein kinase kinase 1; SAPK kinase 1; SAPKK-1; SAPKK1; c-Jun N-terminal kinase kinase 1; JNKK
Protein Family:	Dual specificity mitogen-activated protein kinase kinase
UniProt Gene Name:	MAP2K4
UniProt Entry Name:	MP2K4_HUMAN

	Western blot analysis of extracts of various cell lines, using MAP2K4 antibody (CAB7724) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human kidney using MAP2K4 antibody (CAB7724) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using MAP2K4 antibody (CAB7724) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug MAP2K4 antibody (CAB7724). Western blot was performed from the immunoprecipitate using MAP2K4 antibody (CAB7724) at a dilition of 1:1000.

Antibodies	ELISA Kits
Anti-MAP2K4 Antibody (CAB14781)[KO Validated]	SEK1/MKK4 (Phospho-Thr261) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-MAP2K4-S80 Antibody (CABP0067)	SEK1/MKK4 Colorimetric Cell-Based ELISA Kit
Anti-Phospho-MAP2K4-S257 Antibody (CABP0068)	SEK1/MKK4 (Phospho-Ser80) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-MAP2K4-T261 Antibody (CABP0069)
Anti-Phospho-MAP2K4-S80 Antibody (CABP0390)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)