Anti-MAPK6 Antibody (CAB15304)

Antibody Name:	Anti-MAPK6 Antibody [KO Validated]
Antibody SKU:	CAB15304
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 372-721 of human MAPK6 (NP_002739.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, A549, LO2, BxPC-3, 293T, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 372-721 of human MAPK6 (NP_002739.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NFDI DEVQ LDPR ALSD VTDE EEVQ VDPR KYLD GDRE KYLE DPAF DTNY STEP CWQY SDHH ENKY CDLE CSHT CNYK TRSS SYLD NLVW RESE VNHY YEPK LIID LSNW KEQS KEKS DKKG KSKC ERNG LVKA QIAL EEAS QQLA GKER EKNQ GFDF DSFI AGTI QLSS QHEP TDVV DKLN DLNS SVSQ LELK SLIS KSVS QEKQ EKGM ANLA QLEA LYQS SWDS QFVS GGED CFFI NQFC EVRK DEQV EKEN TYTS YLDK FFSR KEDT EMLE TEPV EDGK LGER GHEE GFLN NSGE FLFN KQLE SIGI PQFH SPVG SPLK SIQA TLTP SAMK SSPQ IPHQ TYSS ILKH LN
Gene ID:	5597
Uniprot:	Q16659
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	82kDa
Observed MW:	120kDa

Synonyms:

MAPK6, ERK3, HsT17250, PRKM6, p97MAPK

Background:

The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.

UniProt Protein Function:	ERK3: a protein kinase of the MAPK family. Plays a critical role in the regulation of cell growth and differentiation. Localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters.
UniProt Protein Details:	Protein type:Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Kinase, protein; EC 2.7.11.24; CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily Chromosomal Location of Human Ortholog: 15q21 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:MAP kinase activity; protein binding; protein serine/threonine kinase activity Biological Process: protein amino acid phosphorylation; signal transduction
NCBI Summary:	The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008]
UniProt Code:	Q16659
NCBI GenInfo Identifier:	2499596
NCBI Gene ID:	5597
NCBI Accession:	Q16659.1
UniProt Secondary Accession:	Q16659,Q68DH4, Q8IYN8, B2R945, B5BU65,
UniProt Related Accession:	Q16659
Molecular Weight:	82,681 Da
NCBI Full Name:	Mitogen-activated protein kinase 6
NCBI Synonym Full Names:	mitogen-activated protein kinase 6
NCBI Official Symbol:	MAPK6
NCBI Official Synonym Symbols:	ERK3; PRKM6; p97MAPK; HsT17250
NCBI Protein Information:	mitogen-activated protein kinase 6
UniProt Protein Name:	Mitogen-activated protein kinase 6
UniProt Synonym Protein Names:	Extracellular signal-regulated kinase 3; ERK-3; MAP kinase isoform p97; p97-MAPK
UniProt Gene Name:	MAPK6
UniProt Entry Name:	MK06_HUMAN

	Western blot analysis of extracts of various cell lines, using MAPK6 antibody (CAB15304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Western blot analysis of extracts from normal (control) and MAPK6 knockout (KO) HeLa cells, using MAPK6 antibody (CAB15304) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunofluorescence analysis of L929 cells using MAPK6 Polyclonal Antibody (CAB15304) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-Phospho-ERK3-S189 Antibody (CABP0051)	ERK3 Colorimetric Cell-Based ELISA Kit
MAPK6 Antibody (PACO00778)	ERK3 (Phospho-Ser189) Colorimetric Cell-Based ELISA Kit
Phospho-MAPK6 (S189) Antibody (PACO04758)
MAPK6 Antibody (PACO04759)
MAPK6 Antibody (PACO07004)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)