The MCM3 Monoclonal Antibody (CAB11475) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human MCM3 and has been validated for use in Western blot applications. By binding to the MCM3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an invaluable resource for studies in molecular biology and cancer research.MCM3 is a critical component of the MCM complex, which plays a crucial role in DNA replication and ensuring genomic stability.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MCM3 Monoclonal Antibody
SKU:
CAB11475
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0607
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HCC5, P1.h, RLFB, P1-MCM3, MCM3
Positive Sample:
HeLa, K-562, C6, Mouse spleen
Cellular Localization:
Nucleus.
Calculated MW:
91kDa
Observed MW:
100kDa
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
4172
RRID
AB_2861574
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MCM3 Rabbit mAb (CAB11475) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from Mouse spleen, using MCM3 Rabbit mAb (CAB11475) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Rat spleen using MCM3 Rabbit mAb (CAB11475) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using MCM3 Rabbit mAb (CAB11475) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg MCM3 antibody (CAB11475). Western blot was performed from the immunoprecipitate using MCM3 antibody (CAB11475) at a dilution of 1:1000.
