null

Anti-MGST1 Antibody (CAB16399)

SKU:
CAB16399
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-MGST1 Antibody
Antibody SKU:CAB16399
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MGST1 (NP_001247440.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse lung
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MGST1 (NP_001247440.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVDL TQVM DDEV FMAF ASYA TIIL SKMM LMST ATAF YRLT RKVF ANPE DCVA FGKG ENAK KYLR TDDR VERV RRAH LNDL ENII PFLG IGLL YSLS GPDP
Gene ID:4257
Uniprot:P10620
Cellular Location:Endoplasmic reticulum membrane, Microsome, Mitochondrion outer membrane, Multi-pass membrane protein, Peripheral membrane protein
Calculated MW:9kDa/17kDa
Observed MW:18kDa
Synonyms:MGST1, GST12, MGST, MGST-I
Background:The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene.
UniProt Protein Function:MGST1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity. Belongs to the MAPEG family.
UniProt Protein Details:

Protein type:Xenobiotic Metabolism - metabolism by cytochrome P450; Mitochondrial; EC 2.5.1.18; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Membrane protein, multi-pass; Membrane protein, integral; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 12p12.3-p12.1

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; mitochondrion; peroxisomal membrane

Molecular Function:glutathione peroxidase activity; glutathione transferase activity; protein binding

Biological Process: xenobiotic metabolic process

NCBI Summary:The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene. [provided by RefSeq, May 2012]
UniProt Code:P10620
NCBI GenInfo Identifier:121740
NCBI Gene ID:4257
NCBI Accession:P10620.1
UniProt Secondary Accession:P10620,A8K533, G5EA53,
UniProt Related Accession:P10620
Molecular Weight:9,984 Da
NCBI Full Name:Microsomal glutathione S-transferase 1
NCBI Synonym Full Names:microsomal glutathione S-transferase 1
NCBI Official Symbol:MGST1  
NCBI Official Synonym Symbols:MGST; GST12; MGST-I  
NCBI Protein Information:microsomal glutathione S-transferase 1
UniProt Protein Name:Microsomal glutathione S-transferase 1
UniProt Synonym Protein Names:Microsomal GST-I
Protein Family:Microsomal glutathione S-transferase
UniProt Gene Name:MGST1  
UniProt Entry Name:MGST1_HUMAN
Anti-MGST1 Antibody (CAB16399)Western blot analysis of extracts of mouse lung, using MGST1 antibody (CAB16399) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.

Related Products