Anti-MMP13 Antibody (CAB16920)

Antibody Name:	Anti-MMP13 Antibody [KO Validated]
Antibody SKU:	CAB16920
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 262-471 of human MMP13 (NP_002418.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, MCF7, A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 262-471 of human MMP13 (NP_002418.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IQSL YGPG DEDP NPKH PKTP DKCD PSLS LDAI TSLR GETM IFKD RFFW RLHP QQVD AELF LTKS FWPE LPNR IDAA YEHP SHDL IFIF RGRK FWAL NGYD ILEG YPKK ISEL GLPK EVKK ISAA VHFE DTGK TLLF SGNQ VWRY DDTN HIMD KDYP RLIE EDFP GIGD KVDA VYEK NGYI YFFN GPIQ FEYS IWSN RIVR VMPA NSIL WC
Gene ID:	4322
Uniprot:	P45452
Cellular Location:	Secreted, extracellular matrix, extracellular space
Calculated MW:	53kDa
Observed MW:	70KDa/55KDa

Synonyms:

MMP13, CLG3, MANDP1, MDST, MMP-13

Background:

This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11.

UniProt Protein Function:	MMP13: Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO). A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age. Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1). Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. Belongs to the peptidase M10A family.
UniProt Protein Details:	Protein type:Secreted; EC 3.4.24.-; Secreted, signal peptide; Protease Chromosomal Location of Human Ortholog: 11q22.3 Cellular Component: extracellular region Molecular Function:calcium ion binding; collagen binding; metalloendopeptidase activity; serine-type endopeptidase activity; zinc ion binding Biological Process: collagen catabolic process; extracellular matrix disassembly Disease: Spondyloepimetaphyseal Dysplasia, Missouri Type
NCBI Summary:	This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11. [provided by RefSeq, Jan 2016]
UniProt Code:	P45452
NCBI GenInfo Identifier:	1168998
NCBI Gene ID:	4322
NCBI Accession:	P45452.1
UniProt Secondary Accession:	P45452,Q6NWN6, A8K846, B2RCZ3,
UniProt Related Accession:	P45452
Molecular Weight:	53,820 Da
NCBI Full Name:	Collagenase 3
NCBI Synonym Full Names:	matrix metallopeptidase 13
NCBI Official Symbol:	MMP13
NCBI Official Synonym Symbols:	CLG3; MANDP1; MMP-13
NCBI Protein Information:	collagenase 3
UniProt Protein Name:	Collagenase 3
UniProt Synonym Protein Names:	Matrix metalloproteinase-13; MMP-13
Protein Family:	Collagenase
UniProt Gene Name:	MMP13
UniProt Entry Name:	MMP13_HUMAN

	Western blot analysis of extracts of various cell lines, using MMP13 antibody (CAB16920) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Western blot analysis of extracts from normal (control) and MMP13 knockout (KO) 293T cells, using MMP13 antibody (CAB16920) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunofluorescence analysis of rat trachea using MMP13 Rabbit pAb (CAB16920) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-MMP13 Antibody (CAB11755)	Human MMP-13 (Matrix Metalloproteinase 13) CLIA Kit (HUES00121)
Anti-MMP13 Antibody (CAB1606)	Human Collagenase 3 (MMP13) ELISA Kit
Anti-MMP13 Antibody (CAB11148)	MMP-13 Colorimetric Cell-Based ELISA Kit
MMP13 Antibody (PACO06645)
MMP13 Antibody (PACO10522)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)