The MonoMethyl-Histone H2B-K5 Antibody (CAB17552) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, possesses high specificity and sensitivity for detecting the monomethylated form of Histone H2B at lysine 5 in human samples.Histone modifications, such as monomethylation of H2B K5, play a crucial role in gene expression regulation and chromatin structure dynamics. The CAB17552 antibody enables precise detection and analysis of this specific histone modification, allowing researchers to investigate its impact on cellular processes and potential involvement in diseases like cancer and developmental disorders.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
MonoMethyl-Histone H2B-K5 Antibody
SKU:
CAB17552
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PAKS A
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:100 - 1:500
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.
Purification Method
Affinity purification
Gene ID
3017 8349
RRID
AB_2770388
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells, using MonoMethyl-Histone H2B-K5 Rabbit pAb (CAB17552) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
Immunofluorescence analysis of C6 cells using MonoMethyl-Histone H2B-K5 Rabbit pAb (CAB17552) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H2B-K5 Rabbit pAb (CAB17552) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using MonoMethyl-Histone H2B-K5 Rabbit pAb (CAB17552) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
