The Mouse anti TAP-Tag Monclonal Antibody (CABE021) is a high-quality antibody developed for reliable detection and analysis of target proteins. The TAP (Tandem Affinity Purification) tag combines two distinct affinity domains separated by a protease cleavage site, enabling sequential purification of tagged proteins and their interaction partners under near-native conditions. This dual-affinity design greatly reduces non-specific contaminants, making it a powerful tool for isolating protein complexes for interaction proteomics.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Species independent samples.
Product Name:
Mouse anti TAP-Tag Monclonal Antibody
SKU:
CABE021
Size:
50μL, 100μL, 200μL
Reactivity:
Species independent
Clone Number:
AMC0510
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:5000 - 1:10000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TAP, TAP tag, TAP-tag
Positive Sample:
Recombinant TAP protein
Observed MW:
75kDa(RecombinantTAPprotein)
Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.
Purification Method
Affinity purification
RRID
AB_2770411
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of recombinant TAP protein using mouse anti TAP-Tag mAb (CABE021) at dilution of 1:5000. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
