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Anti-Mouse anti V5-Tag Monoclonal Antibody (CABE017)

SKU:
CABE017
Product Type:
Antibody
Host Species:
Mouse
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
€349
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Mouse anti V5-Tag Monoclonal Antibody
Antibody SKU:CABE017
Antibody Size:100 uL
Application:WB IF IP
Reactivity:
Host Species:Mouse
Immunogen:A synthetic peptide corresponding to V5 tag.
Application:WB IF IP
Recommended Dilution:WB 1:1000 - 1:5000 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:
Positive Samples:293T
Immunogen:A synthetic peptide corresponding to V5 tag.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GKPI PNPL LGLD ST
Gene ID:
Uniprot:
Cellular Location:
Calculated MW:
Observed MW:Refer to Figures
Anti-Mouse anti V5-Tag Monoclonal Antibody (CABE017)Western blot analysis of recombinant V5 protein using Mouse anti V5-Tag mAb (CABE017) at dilution of 1:2000. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (CABS003) at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:V5, V5 tag, V5-tag
Background:Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

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