The Mouse B-Cell CLL/Lymphoma 9 (BCL9) ELISA Kit is specifically designed for the accurate detection of BCL9 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.BCL9 is a critical protein involved in B-cell lymphoma and is essential for cell proliferation and survival. It plays a significant role in the development and progression of B-cell CLL/Lymphoma, making it a valuable biomarker for studying this condition and exploring potential therapeutic options.
With this ELISA kit, researchers can confidently measure BCL9 levels in mouse samples, providing valuable insights into the molecular mechanisms underlying B-cell CLL/Lymphoma and potentially identifying novel targets for therapeutic intervention. Order your Mouse BCL9 ELISA Kit today to advance your research in this important area.
Product Name:
Mouse B-Cell CLL/Lymphoma 9 (Bcl9) ELISA Kit
Product Code:
MODL00149
Size:
96 Assays
Target:
Bcl9
Synonyms:
LGS, Protein legless homolog
Detection Method:
Sandwich
Range:
78.125-5000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
90-101
95
Heparin plasma (n=5)
91-102
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
