The Mouse Gal3 (Galactin-3) CLIA Kit is a reliable and accurate tool designed for the detection of Galactin-3 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it suitable for a variety of research applications.Galactin-3, also known as Mac-2 antigen, is a multifunctional protein involved in cell adhesion, proliferation, and apoptosis.
It plays a crucial role in inflammation, fibrosis, and cancer progression, making it a valuable biomarker for studying these conditions and exploring potential therapeutic strategies.Get your Mouse Gal3 CLIA Kit today and unlock the potential of studying the role of Galactin-3 in various physiological and pathological processes.
Product Name:
Mouse GAL3 (Galectin 3) CLIA Kit
SKU:
MOES00276
Target:
Mouse GAL3 (Galectin 3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GAL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAL3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GAL3. You can calculate the concentration of Mouse GAL3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
100-114
94-107
Average (%)
108
107
101
1:4
Range (%)
99-113
88-100
85-98
Average (%)
107
93
91
1:8
Range (%)
98-114
94-109
101-116
Average (%)
104
102
107
1:16
Range (%)
100-113
97-112
85-98
Average (%)
105
103
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
92-107
98
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.13
69.32
386.86
37.92
66.93
392.57
Standard deviation
3.07
8.01
30.72
4.67
5.15
40.91
C V (%)
8.27
11.56
7.94
12.32
7.69
10.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GAL3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GAL3 in samples. No significant cross-reactivity or interference between Mouse GAL3 and analogues was observed.
