The Mouse IgG2b (Immunoglobulin G2b) ELISA Kit is a highly reliable and sensitive tool designed for the accurate detection of IgG2b levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional specificity and precision, ensuring trustworthy and reproducible results suitable for a variety of research applications.Immunoglobulin G2b is a critical component of the immune system, playing a key role in antibody-mediated responses and immune regulation. It is involved in various immune processes, including immune response modulation and inflammation regulation, making it a valuable biomarker for studying immunological conditions and developing potential therapies.Overall, the Mouse IgG2b (Immunoglobulin G2b) ELISA Kit is an essential tool for researchers looking to accurately measure IgG2b levels in mouse samples and further understand the immune system's functions and responses.
Product Name:
Mouse IgG2b (Immunoglobulin G2b) ELISA Kit
SKU:
MOES01680
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
