The Mouse IL-10 ELISA Kit is specially designed for the quantitative detection of interleukin-10 (IL-10) in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reproducible results for a variety of research applications.IL-10 is a key immunoregulatory cytokine that plays a crucial role in modulating immune responses and inflammation. It is involved in regulating T cell differentiation, B cell function, and macrophage activation. Dysregulation of IL-10 has been linked to various autoimmune diseases, inflammatory disorders, and infections, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.Overall, the Mouse IL-10 ELISA Kit provides researchers with a powerful tool for studying the role of IL-10 in immune responses and inflammation, offering valuable insights for advancing our understanding of disease mechanisms and developing novel treatment approaches.
Product Name:
Mouse IL-10 ELISA Kit
SKU:
MOES00665
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
