The Mouse IL-18 CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of IL-18 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.IL-18 is a pro-inflammatory cytokine that plays a key role in the immune response and the regulation of inflammation. Elevated levels of IL-18 have been associated with various inflammatory diseases, autoimmune conditions, and metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential therapies. With its high sensitivity and specificity, the Mouse IL-18 CLIA Kit offers researchers a powerful tool for studying the role of IL-18 in various disease processes and for evaluating potential therapeutic interventions. Order now to advance your research with confidence and precision.
Product Name:
Mouse IL-18 CLIA Kit
SKU:
MOES00411
Size:
96 Assays
Detection Method:
Chemiluminescence
Assay type:
Sandwich-CLIA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This kit uses a sandwich chemiluminescence immunoassay (CLIA) principle. The microplate is pre-coated with an antibody specific to the target protein. Standards or samples are added to the wells and bind to the immobilized antibody. A biotinylated detection antibody is then added, followed by HRP-conjugated streptavidin to form a sandwich complex. After washing to remove unbound components, a chemiluminescent substrate is added. The HRP enzyme catalyzes a light-emitting reaction. The intensity of the emitted light is directly proportional to the concentration of the target protein in the sample. The signal is measured using a luminometer, and the concentration of the analyte is calculated based on a standard curve.
