The Mouse IL-18 CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of IL-18 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.IL-18 is a pro-inflammatory cytokine that plays a key role in the immune response and the regulation of inflammation. Elevated levels of IL-18 have been associated with various inflammatory diseases, autoimmune conditions, and metabolic disorders, making it a valuable biomarker for studying these conditions and developing potential therapies.
With its high sensitivity and specificity, the Mouse IL-18 CLIA Kit offers researchers a powerful tool for studying the role of IL-18 in various disease processes and for evaluating potential therapeutic interventions. Order now to advance your research with confidence and precision.
Product Name:
Mouse IL-18 CLIA Kit
SKU:
MOES00411
Target:
Mouse IL-18
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-18. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-18, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-18. You can calculate the concentration of Mouse IL-18 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
101-117
97-111
Average (%)
100
109
104
1:4
Range (%)
95-108
90-101
97-112
Average (%)
100
95
104
1:8
Range (%)
91-103
92-108
100-116
Average (%)
96
99
106
1:16
Range (%)
89-104
96-110
92-107
Average (%)
95
102
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-114
107
EDTA plasma (n=5)
83-96
90
Cell culture media (n=5)
98-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.28
67.16
200.74
26.74
71.88
207.46
Standard deviation
2.3
8.03
14.23
3.45
6.18
15.5
C V (%)
9.1
11.96
7.09
12.9
8.6
7.47
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-18 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-18 in samples. No significant cross-reactivity or interference between Mouse IL-18 and analogues was observed.
