Mouse IL36G Recombinant Protein (RPPB0687)
- SKU:
- RPPB0687
- Product Type:
- Recombinant Protein
- Species:
- Mouse
- Uniprot:
- Q8R460
- Research Area:
- Cytokines
Description
| Product Name: | Mouse IL36G Recombinant Protein |
| Product Code: | RPPB0687 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | IL36G |
| Synonyms: | Interleukin-36 gamma, Interleukin-1 family member 9, IL-1F9, Il36g, Il1f9. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution in 1M MOPS, 10 mM NaAC, pH7.6, 5 % Trehalose, 2 mM EDTA and 0.02 % Tween-20. |
| Solubility: | It is recommended to reconstitute the lyophilized IL36g in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized IL36g�Mouse although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL36g should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | GRETPDFGEV FDLDQQVWIF RNQALVTVPR SHRVTPVSVT ILPCKYPESL EQDKGIAIYL GIQNPDKCLF CKEVNGHPTL LLKEEKILDL YHHPEPMKPF LFYHTRTGGT STFESVAFPG HYIASSKTGN PIFLTSKKGE YYNINFNLDI KS |
| Biological Activity: | The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0�105 IU/mg. |
IL-36gamma belongs to the IL-1 family which includes IL-1b, IL-1a, IL-1ra, IL-18, IL-36 Ra (IL-1F5), IL-36a (IL-1F6), IL-36b (IL-1F8), IL-37 (IL-1F7) and IL-1F10. ). The IL-1 family members display a 12 b-strand, b-trefoil configuration, and are thought to have ascended from a mutual ancestral gene. IL-36g is an 18-22 kDa, 169aa intracellular and secreted protein which holds no signal sequence, no prosegment and no potential N-linked glycosylation sites. Human IL-36g shares 58%- 69% aa sequence homology with mouse, rat, bovine and equine IL-36g, and 23 - 57% aa sequence homology with other family members. The IL-36g receptor is a mixture of IL-1 Rrp2, mostly located in epithelia and keratinocytes, and the extensively expressed IL-1 RAcP. All IL-36 (a, b and g) activate N F-kB and MAPK pathways in an IL-1 Rrp2 dependent reaction. Additionally, IL-36g induces production of inflammatory cytokines and chemokines like CXCL8/IL-8.
IL36G Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 152 amino acids and having a molecular mass of 17.3kDa.The IL36G is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Functions as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2/IL1RL2. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP (). Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. May play a role in proinflammatory responses during particular neutrophilic airway inflammation. May be involved in the innate immune response to fungal pathogens. Induces the production of proinflammatory cytokines in bone marrow-derived dendritic cells (BMDCs), including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23. Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4+ T-cells and splenocytes. |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q8R460 |
| NCBI GenInfo Identifier: | 25008588 |
| NCBI Gene ID: | 215257 |
| NCBI Accession: | Q8R460.1 |
| UniProt Secondary Accession: | Q8R460,Q14BB6 |
| UniProt Related Accession: | Q8R460 |
| Molecular Weight: | |
| NCBI Full Name: | Interleukin-36 gamma |
| NCBI Synonym Full Names: | interleukin 1 family, member 9 |
| NCBI Official Symbol: | Il1f9�� |
| NCBI Official Synonym Symbols: | Il36g�� |
| NCBI Protein Information: | interleukin-36 gamma |
| UniProt Protein Name: | Interleukin-36 gamma |
| UniProt Synonym Protein Names: | Interleukin-1 family member 9; IL-1F9 |
| Protein Family: | |
| UniProt Gene Name: | Il36g�� |
| UniProt Entry Name: |
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