SynonymsNRP,FIP2,GLC1E,HIP7,HYPL,TFIIIA-INTP,Glaucoma 1,Open Angle,E(Adult-Onset),Huntingtin-interacting protein 7,Optic neuropathy-inducing protein,E3-14.7K-interacting protein
ReactivityMouse
Sample TypeTissue Homogenates And Other Biological Fluids
SKU:
MODL00991
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8K3K8
Sensitivity:
0.051ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
NRP,FIP2,GLC1E,HIP7,HYPL,TFIIIA-INTP,Glaucoma 1,Open Angle,E(Adult-Onset),Huntingtin-interacting protein 7,Optic neuropathy-inducing protein,E3-14.7K-interacting protein
The Mouse Optineurin (OPTN) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of optineurin levels in mouse serum, plasma, and cell culture supernatants. Optineurin is a key protein involved in various cellular processes, including autophagy, inflammation, and immunity.This ELISA kit offers reliable and reproducible results, making it suitable for a wide range of research applications.
Optineurin's role in neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS) and glaucoma, makes it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With easy-to-follow protocols and quick assay times, the Mouse Optineurin (OPTN) ELISA Kit is an essential tool for researchers investigating optineurin's function and significance in health and disease.
Product Name:
Mouse Optineurin (OPTN) ELISA Kit
Product Code:
MODL00991
Size:
96 Assays
Target:
OPTN
Synonyms:
NRP, FIP2, GLC1E, HIP7, HYPL, TFIIIA-INTP, Glaucoma 1,Open Angle,E(Adult-Onset), Huntingtin-interacting protein 7, Optic neuropathy-inducing protein, E3-14.7K-interacting protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
