The Mouse Protein Kinase C Epsilon (PKCε) ELISA Kit is a reliable and sensitive assay designed for the accurate measurement of PKCε levels in mouse samples, including serum, plasma, and tissue homogenates. This kit offers high specificity and precision, ensuring consistent and dependable results for various research applications.PKCε is a key protein kinase involved in cell signaling pathways, regulating processes such as cell growth, differentiation, and apoptosis.
Dysregulation of PKCε has been linked to various diseases, including cancer, cardiovascular disorders, and neurological conditions. Monitoring PKCε levels can provide valuable insights into these diseases and aid in the development of targeted therapies.Overall, the Mouse PKCε ELISA Kit from Assay Genie is an essential tool for researchers studying PKCε-related pathways and diseases, offering reliable and accurate quantification of PKCε levels in mouse samples.
Product Name:
Mouse Protein Kinase C Epsilon (PKCe) ELISA Kit
Product Code:
MODL01054
Size:
96 Assays
Target:
PKCe
Synonyms:
PKC-E, PRKCE, PRKC-E, NPKC-Epsilon
Detection Method:
Competition
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
