The Mouse TG (Thyroglobulin) CLIA Kit is specially designed for the quantitative measurement of thyroglobulin levels in mouse serum, plasma, and cell culture supernatants. This kit offers high precision and accuracy, allowing for consistent and reliable results that are essential for various research purposes.Thyroglobulin is a key protein involved in thyroid function, serving as a precursor to thyroid hormone production.
Abnormal thyroglobulin levels can indicate thyroid disorders, including thyroid cancer and autoimmune conditions. This makes the Mouse TG CLIA Kit a valuable tool for studying thyroid function, monitoring disease progression, and evaluating treatment effectiveness.
Product Name:
Mouse TG (Thyroglobulin) CLIA Kit
SKU:
MOES00561
Target:
Mouse TG (Thyroglobulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse TG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse TG. You can calculate the concentration of Mouse TG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
91-104
91-106
Average (%)
108
99
98
1:4
Range (%)
102-116
96-108
100-114
Average (%)
109
101
108
1:8
Range (%)
91-103
87-102
93-109
Average (%)
97
93
101
1:16
Range (%)
102-118
94-108
87-99
Average (%)
108
101
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
89-100
95
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.42
241.27
835.89
109.97
225.36
855.25
Standard deviation
10.59
17.08
78.07
10.48
19.7
70.9
C V (%)
10.55
7.08
9.34
9.53
8.74
8.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse TG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TG in samples. No significant cross-reactivity or interference between Mouse TG and analogues was observed.
