The Mouse TXB2 (Thromboxane B2) ELISA Kit is a powerful tool for quantifying thromboxane B2 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, ensuring precise and accurate results for a variety of research applications.Thromboxane B2 is a key metabolite of thromboxane A2, a potent mediator of platelet aggregation and vasoconstriction. High levels of thromboxane B2 have been associated with cardiovascular diseases, inflammation, and other pathological conditions. By measuring thromboxane B2 levels, researchers can gain valuable insights into these processes and potentially identify new therapeutic targets.Whether studying the mechanisms of thromboxane signaling or investigating the role of thromboxane in disease pathology, the Mouse TXB2 (Thromboxane B2) ELISA Kit is an indispensable tool for researchers seeking to advance our understanding of these complex biological processes.
Product Name:
Mouse TXB2 (Thromboxane B2) ELISA Kit
SKU:
MOES01550
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
