Anti-MYH9 Antibody (CAB16923)

Antibody Name:	Anti-MYH9 Antibody
Antibody SKU:	CAB16923
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1711-1960 of human MYH9 (NP_002464.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HT-29, A-549, NIH/3T3, C6

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1711-1960 of human MYH9 (NP_002464.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ANSS GKGA LALE EKRR LEAR IAQL EEEL EEEQ GNTE LIND RLKK ANLQ IDQI NTDL NLER SHAQ KNEN ARQQ LERQ NKEL KVKL QEME GTVK SKYK ASIT ALEA KIAQ LEEQ LDNE TKER QAAC KQVR RTEK KLKD VLLQ VDDE RRNA EQYK DQAD KAST RLKQ LKRQ LEEA EEEA QRAN ASRR KLQR ELED ATET ADAM NREV SSLK NKLR RGDL PFVV PRRM ARKG AGDG SDEE VDGK ADGA EAKP AE
Gene ID:	4627
Uniprot:	P35579
Cellular Location:	Cytoplasm, cell cortex, cytoskeleton
Calculated MW:	159kDa/226kDa
Observed MW:	250KDa

Synonyms:

MYH9, BDPLT6, DFNA17, EPSTS, FTNS, MHA, NMHC-II-A, NMMHC-IIA, NMMHCA, myosin-9

Background:

This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.

UniProt Protein Function:	MYH9: Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Interacts with PDLIM2. Interacts with SLC6A4. Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Interacts with RASIP1. Interacts with DDR1. Interacts with SVIL and HTRA3. In the kidney, expressed in the glomeruli. Also expressed in leukocytes. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motor; Motility/polarity/chemotaxis; Actin-binding Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: actin cytoskeleton; actomyosin; cleavage furrow; contractile ring; cytoplasm; cytosol; focal adhesion; immunological synapse; integrin complex; leading edge; membrane; myosin II complex; nucleus; plasma membrane; protein complex; ruffle; signalosome; stress fiber; uropod Molecular Function:actin binding; actin filament binding; actin-dependent ATPase activity; ADP binding; ATP binding; ATPase activity; microfilament motor activity; protein anchor; protein binding; protein domain specific binding; protein homodimerization activity Biological Process: actin cytoskeleton reorganization; actin filament-based movement; actomyosin structure organization and biogenesis; angiogenesis; blood vessel endothelial cell migration; cytokinesis; membrane protein ectodomain proteolysis; monocyte differentiation; phagocytosis, engulfment; platelet formation; protein transport; regulation of cell shape Disease: Deafness, Autosomal Dominant 17; Epstein Syndrome; Fechtner Syndrome; Macrothrombocytopenia And Progressive Sensorineural Deafness; May-hegglin Anomaly; Sebastian Syndrome
NCBI Summary:	This gene encodes a conventional non-muscle myosin; this protein should not be confused with the unconventional myosin-9a or 9b (MYO9A or MYO9B). The encoded protein is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain which is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in this gene have been associated with non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness. [provided by RefSeq, Dec 2011]
UniProt Code:	P35579
NCBI GenInfo Identifier:	6166599
NCBI Gene ID:	4627
NCBI Accession:	P35579.4
UniProt Secondary Accession:	P35579,O60805, Q60FE2, Q86T83, A8K6E4,
UniProt Related Accession:	P35579
Molecular Weight:	159,864 Da
NCBI Full Name:	Myosin-9
NCBI Synonym Full Names:	myosin, heavy chain 9, non-muscle
NCBI Official Symbol:	MYH9
NCBI Official Synonym Symbols:	MHA; FTNS; EPSTS; BDPLT6; DFNA17; NMMHCA; NMHC-II-A; NMMHC-IIA
NCBI Protein Information:	myosin-9
UniProt Protein Name:	Myosin-9
UniProt Synonym Protein Names:	Cellular myosin heavy chain, type A; Myosin heavy chain 9; Myosin heavy chain, non-muscle IIa; Non-muscle myosin heavy chain A; NMMHC-A; Non-muscle myosin heavy chain IIa; NMMHC II-a; NMMHC-IIA
Protein Family:	Myosin
UniProt Gene Name:	MYH9
UniProt Entry Name:	MYH9_HUMAN

	Western blot analysis of extracts of various cell lines, using MYH9 antibody (CAB16923) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat kidney using MYH9 Rabbit pAb (CAB16923) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human thyroid cancer using MYH9 Rabbit pAb (CAB16923) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using MYH9 Rabbit pAb (CAB16923) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-MYH9 Antibody (CAB0173)	Human MYH9 / Myosin-9 ELISA Kit
Anti-Phospho-MYH9-S1943 pAb Antibody (CABP0802)	Human MYH9 (Myosin Heavy Chain 9, Non Muscle) CLIA Kit (HUES00457)
MYH9 Antibody (PACO10683)	Human MYH9 (Myosin Heavy Chain 9, Non Muscle) ELISA Kit (HUES01870)
	Human Myosin-9 (MYH9) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)