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Anti-NEK2 Antibody (CAB13334)

SKU:
CAB13334
Product Type:
Antibody
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
  • Cell Cycle Antibodies 1 Anti-NEK2 Antibody CAB13334
  • Cell Cycle Antibodies 1 Anti-NEK2 Antibody CAB13334
€139 - €419
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Description

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Antibody Name:Anti-NEK2 Antibody
Antibody SKU:CAB13334
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human NEK2 (NP_002488.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Rat
Positive Samples:HeLa
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human NEK2 (NP_002488.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SPVL SELK LKEI QLQE RERA LKAR EERL EQKE QELC VRER LAED KLAR AENL LKNY SLLK ERKF LSLA SNPE LLNL PSSV IKKK VHFS GESK ENIM RSEN SESQ LTSK SKCK DLKK RLHA AQLR AQAL SDIE KNYQ LKSR QILG MR
Gene ID:4751
Uniprot:P51955
Cellular Location:Chromosome, Cytoplasm, Nucleus, centromere, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, nucleolus, spindle pole
Calculated MW:37kDa/44kDa/50kDa/51kDa
Observed MW:52kDa
Synonyms:NEK2, HsPK21, NEK2A, NLK1, PPP1R111, RP67
Background:This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene.
UniProt Protein Function:NEK2: a protein kinase of the NEK family. Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation
UniProt Protein Details:

Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Nucleolus; Protein kinase, Other; EC 2.7.11.1; Other group; NEK family

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: kinetochore; spindle pole; microtubule; centrosome; intercellular bridge; protein complex; condensed nuclear chromosome; cytoplasm; nucleolus; midbody; nucleus; cytosol

Molecular Function:protein serine/threonine kinase activity; protein binding; metal ion binding; protein phosphatase binding; ATP binding; protein kinase activity

Biological Process: mitosis; blastocyst development; regulation of attachment of spindle microtubules to kinetochore; mitotic sister chromatid segregation; protein amino acid autophosphorylation; regulation of mitotic centrosome separation; organelle organization and biogenesis; centrosome separation; spindle assembly; protein amino acid phosphorylation; negative regulation of DNA binding; chromosome segregation; meiotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; regulation of mitosis; mitotic cell cycle; G2/M transition of mitotic cell cycle

Disease: Retinitis Pigmentosa 67

NCBI Summary:This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]
UniProt Code:P51955
NCBI GenInfo Identifier:1709252
NCBI Gene ID:4751
NCBI Accession:P51955.1
UniProt Secondary Accession:P51955,Q53FD6, Q5I1Z9, Q5VXZ1, Q6NZX8, Q7Z634, Q86XH2 Q96QN9,
UniProt Related Accession:P51955
Molecular Weight:445
NCBI Full Name:Serine/threonine-protein kinase Nek2
NCBI Synonym Full Names:NIMA-related kinase 2
NCBI Official Symbol:NEK2  
NCBI Official Synonym Symbols:NLK1; RP67; NEK2A; HsPK21; PPP1R111  
NCBI Protein Information:serine/threonine-protein kinase Nek2; nimA-like protein kinase 1; nimA-related protein kinase 2; protein phosphatase 1, regulatory subunit 111; NIMA (never in mitosis gene a)-related kinase 2
UniProt Protein Name:Serine/threonine-protein kinase Nek2
UniProt Synonym Protein Names:HSPK 21; Never in mitosis A-related kinase 2; NimA-related protein kinase 2; NimA-like protein kinase 1
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:NEK2  
UniProt Entry Name:NEK2_HUMAN
Anti-NEK2 Antibody (CAB13334)Western blot analysis of extracts of HeLa cells, using NEK2 antibody (CAB13334) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-NEK2 Antibody (CAB13334)Immunohistochemistry of paraffin-embedded rat ovary using NEK2 Rabbit pAb (CAB13334) at dilution of 1:100 (40x lens).

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