Description
NF-kappaB p65 (Phospho-Ser276)Transcription Factor Activity Assay
The NF-kappaB p65 (Phospho-Ser276) Transcription Factor Activity Assay Kit is a powerful tool for the accurate measurement of NF-kappaB p65 activity in cell extracts. This kit is specifically designed for the detection of phosphorylated NF-kappaB p65 at serine 276, providing valuable insights into the transcriptional activity of this important transcription factor.By utilizing a highly sensitive and specific assay format, researchers can confidently assess NF-kappaB p65 activity in a variety of sample types, including cell lysates and tissue homogenates. The data obtained from this assay can help uncover the molecular mechanisms underlying inflammatory responses, immune regulation, and disease pathogenesis.
With its easy-to-use protocol and reliable performance, the NF-kappaB p65 (Phospho-Ser276) Transcription Factor Activity Assay Kit is a valuable tool for investigating the role of NF-kappaB signaling in various physiological and pathological processes. Whether studying inflammatory disorders, cancer progression, or autoimmune diseases, this assay kit provides researchers with the precision and accuracy needed for insightful discoveries.
| Product Name: | NF-kappaB p65 (Phospho-Ser276)Transcription Factor Activity Assay |
| Product Code: | TFAB00043 |
| Target: | NF-kappaB p65 (Phospho-Ser276) |
| Synonyms: | Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 |
| Reactivity: | Human, Mouse |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie NF-kappaB p65 (Phospho-Ser276) transcription factor activity assay allows for the detection and qualitative analysis of NF-kappaB p65 (Phospho-Ser276) active transcription factors in eukaryotic nuclear or cell lysates.
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified. |
| UniProt Protein Details: | Protein type:DNA-binding; Nuclear receptor co-regulator; Transcription factor Chromosomal Location of Human Ortholog: 11q13.1 Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex Molecular Function:actinin binding; chromatin binding; chromatin DNA binding; DNA binding; histone deacetylase binding; identical protein binding; NF-kappaB binding; phosphate binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein N-terminus binding; RNA polymerase II transcription factor activity, enhancer binding; transcription activator binding; transcription factor activity; transcription factor binding; ubiquitin protein ligase binding Biological Process: activation of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; inflammatory response; membrane protein intracellular domain proteolysis; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of inflammatory response; response to organic substance; response to UV-B; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway |
| NCBI Summary: | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | Q04206 |
| NCBI GenInfo Identifier: | 62906901 |
| NCBI Gene ID: | 5970 |
| NCBI Accession: | Q04206.2 |
| UniProt Secondary Accession: | Q04206,Q6GTV1, Q6SLK1, |
| UniProt Related Accession: | Q04206 |
| Molecular Weight: | 59,910 Da |
| NCBI Full Name: | Transcription factor p65 |
| NCBI Synonym Full Names: | RELA proto-oncogene, NF-kB subunit |
| NCBI Official Symbol: | RELA |
| NCBI Official Synonym Symbols: | p65; NFKB3 |
| NCBI Protein Information: | transcription factor p65 |
| UniProt Protein Name: | Transcription factor p65 |
| UniProt Synonym Protein Names: | Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 |
| Protein Family: | Proline-rich P65 protein |
| UniProt Gene Name: | RELA |
