Anti-NFS1 Antibody (CAB13385)

Antibody Name:	Anti-NFS1 Antibody
Antibody SKU:	CAB13385
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 208-457 of human NFS1 (NP_066923.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, SKOV3, Mouse kidney, Mouse heart, Rat liver, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 208-457 of human NFS1 (NP_066923.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EIGV KQPI AEIG RICS SRKV YFHT DAAQ AVGK IPLD VNDM KIDL MSIS GHKI YGPK GVGA IYIR RRPR VRVE ALQS GGGQ ERGM RSGT VPTP LVVG LGAA CEVA QQEM EYDH KRIS KLSE RLIQ NIMK SLPD VVMN GDPK HHYP GCIN LSFA YVEG ESLL MALK DVAL SSGS ACTS ASLE PSYV LRAI GTDE DLAH SSIR FGIG RFTT EEEV DYTV EKCI QHVK RLRE MSPL WEMV QDGI DLKS IKWT QH
Gene ID:	9054
Uniprot:	Q9Y697
Cellular Location:	Cytoplasm, Mitochondrion, Nucleus
Calculated MW:	44kDa/50kDa
Observed MW:	50kDa

Synonyms:

NFS1, HUSSY-08, IscS, NIFS

Background:

Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described.

UniProt Protein Function:	NFS1: Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron- sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily. 2 isoforms of the human protein are produced by alternative initiation.
UniProt Protein Details:	Protein type:EC 2.8.1.7; Cofactor and Vitamin Metabolism - thiamine; Transferase; Mitochondrial Chromosomal Location of Human Ortholog: 20q11.22 Cellular Component: nucleoplasm; intracellular membrane-bound organelle; mitochondrion; mitochondrial matrix; cytoplasm; nucleolus; cytosol; nucleus Molecular Function:protein binding; protein homodimerization activity; cysteine desulfurase activity; pyridoxal phosphate binding Biological Process: iron incorporation into metallo-sulfur cluster; cysteine metabolic process; vitamin metabolic process; sulfur amino acid metabolic process; protein complex assembly; Mo-molybdopterin cofactor biosynthetic process; molybdopterin cofactor biosynthetic process; water-soluble vitamin metabolic process
NCBI Summary:	Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010]
UniProt Code:	Q9Y697
NCBI GenInfo Identifier:	62512153
NCBI Gene ID:	9054
NCBI Accession:	Q9Y697.3
UniProt Secondary Accession:	Q9Y697,Q6P0L8, Q9NTZ5, Q9Y481, B3KMA5, B4DXK9, E1P5R8 F5GYK5,
UniProt Related Accession:	Q9Y697
Molecular Weight:	457
NCBI Full Name:	Cysteine desulfurase, mitochondrial
NCBI Synonym Full Names:	NFS1 cysteine desulfurase
NCBI Official Symbol:	NFS1
NCBI Official Synonym Symbols:	IscS; NIFS; HUSSY-08
NCBI Protein Information:	cysteine desulfurase, mitochondrial; NFS1 nitrogen fixation 1 homolog; nitrogen-fixing bacteria S-like protein; nitrogen fixation 1 (S. cerevisiae, homolog)
UniProt Protein Name:	Cysteine desulfurase, mitochondrial
Protein Family:	Cysteine desulfurase
UniProt Gene Name:	NFS1
UniProt Entry Name:	NFS1_HUMAN

	Western blot analysis of extracts of various cell lines, using NFS1 antibody (CAB13385) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded human liver cancer using NFS1 antibody (CAB13385) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of HeLa cells using NFS1 antibody (CAB13385). Blue: DAPI for nuclear staining.

Antibodies

Anti-NFS1 Antibody (CAB6668)

NFS1 Antibody (PACO10838)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)